RNA interference technology : from basic science to drug development / edited by Krisharao Appasani.

Format:

Book

Contributor:

Appasani, Krishnarao, 1959- editor.

Language:

English

Subjects (All):

Small interfering RNA.

Gene silencing.

Physical Description:

1 online resource (xxxiii, 510 pages) : digital, PDF file(s).

Place of Publication:

Cambridge : Cambridge University Press, 2004.

Language Note:

English

Summary:

RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this 2005 overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.

Contents:

Sect. 1. Basic RNAi, siRNA, microRNAs and gene-silencing mechanisms. RNAi beginnings. Overview of the pathway in C. elegans

Dicer in RNAi: its roles in vivo and utility in vitro

Genes required for RNA interference

MicroRNAs: a small contribution from worms

miRNAs in the brain and the application of RNAi to neurons

Sect. 2. Design, synthesis of siRNAs. Degign and synthesis of small interfering RNA (siRNA)

Automated design and high throughput chemical synthesis of siRNA

Rational design of siRNAs with the Sfold software

Enzymatic production of small interfering RNAs

Sect. 3. Vector development and in vivo, in vitro and in ovo delivery methods. Six methods of inducing RNAi in mammalian cells

Viral delivery of shRNA

siRNA delivery by lentiviral vectors: design and applications

Liposomal delivery of siRNAs in mice.

Chemical modifications to achieve increased stability and sensitive detection of siRNA

RNA interference in postimplantation mouse embryos

In ovo RNAi opens new possibilities for functional genomics in vertebrates

Sect. 4. Gene silencing in model organisms. Practical applications of RNAi in C. elegans

Inducible RNAi as a forward genetic tool in Trypanosoma brucei

RNA-mediated gene silencing in fission yeast

RNA silencing in filamentous fungi: Mucor ciccinelloides as a model organism

RNAi and gene silencing phenomena mediated by viral suppressors in plants.

Sect. 5. Drug target validation. Delivering siRNA in vivo for functional genomics and novel therapeutics

The role of RNA interference in drug target validation: application to Hepatitis C

RNAi in the drug discovery process

RNA interference technology in the discovery and validation of druggable targets

Sect. 6. Therapeutic and drug development. RNAi-mediated silencing of viral gene expression and replication

RNAi in drug development: practical considerations

RNA interference studies in liver failure

RNAi applications in living animal systems

Sect. 7. High-throughput genome-wide RNAi analysis. High-throughput RNAi by soaking in Caenorhabtis elegans

Tools for integrative genomics: genome-wide RNAi and expression profiling in Drosophila

Microarray analysis and RNA silencing to determine genes functionally important in mesothelioma

High-throughput RNA interference

Generation of highly specific vector-based shRNAi libraries directed against the entire human genome.

Notes:

Title from publisher's bibliographic system (viewed on 05 Oct 2015).

Includes bibliographical references and index.

ISBN:

1-107-13974-0

1-280-16317-8

9786610163175

0-511-19685-7

0-511-12174-1

0-511-08221-5

0-511-29831-5

0-511-54640-8

0-511-08176-6

OCLC:

122927915