G-quadruplex structures : formation and role in biology / Hannah Santos, editor.

Format:

Book

Contributor:

Santos, Hannah, editor.

Series:

Biochemistry research trends series.

Biochemistry Research Trends

Language:

English

Subjects (All):

Quadruplex nucleic acids.

Physical Description:

1 online resource (227 p.)

Place of Publication:

New York : Nova Publishers, 2016.

Language Note:

English

Summary:

This book provides current research on G-quadruplex structures, their formation and role in biology. Chapter One summarizes the structural features of quadruplexes built by sequence extensions and site-specifically incorporated modified building blocks that have been established to induce the formation of stable, majority single G-quadruplex folding topologies, primarily from the human telomere repeat DNAs. Chapter Two discusses the G-quadruplexes and i-motifs as scaffolds for molecular engineering of DNA aptamers. Chapter Three examines the case of the natural alkaloids coptisine and chelerythrine. Chapter Four describes the G-quadruplex induced fluorescent signals that have been employed for the study of their folding and unfolding mechanism and thereby visualisation of their formation and structure. Chapter Five reviews the historical aspect of how major biological roles of G-quadruplexes have been uncovered by the use of specifically designed small molecule probes; and discusses the future applications of G-quadruplex interacting small molecules, including the development as potential drug candidates. Chapter Six focuses on the latest advances in the development of the telomerase assays and the authors further discuss the application potentiality of the assays for clinical samples.

Contents:

G-QUADRUPLEX STRUCTURES, FORMATION AND ROLE IN BIOLOGY; G-QUADRUPLEX STRUCTURES, FORMATION AND ROLE IN BIOLOGY; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; Chapter 1: DESIGNING G-QUADRUPLEX TOPOLOGIES BY FOCUSING ON SEQUENCE MODIFICATIONS; ABSTRACT; INTRODUCTION; 1. EFFECT OF SEQUENCE ON QUADRUPLEX TOPOLOGY; 1.1. G-rich Sequences That Predispose Majority Folds; 1.2. Effects of Sequence and Length of the Loops on Folding Topology; 2. MUTATIONS OF SEQUENCES BY NATURAL AND SYNTHETIC NUCLEOTIDES THAT STABILIZE SINGLE FOLDS; 2.1. Guanine Analogs in the G-tetrads

2.2. Sugar Analogs of Tetrad Guanosines2.3. Modification of the Sugar-Phosphate Backbone; 2.4. Loops with Nucleotide Analogs; 2.5. Topologically Constrained Quadruplexes; 3. EFFECT OF CATION AND ITS CONCENTRATION ON G-QUADRUPLEX FOLDING; 4. TOPOLOGY CHANGES IN MOLECULAR CROWDING - EFFECT OF COSOLUTES; 5. EFFECT OF HIGH DNA CONCENTRATIONS ON THE FOLDING TOPOLOGY OF QUADRUPLEXES; CONCLUSION; REFERENCES; Chapter 2: G-QUADRUPLEXES AND I-MOTIFS AS SCAFFOLDS FOR MOLECULAR ENGINEERING OF DNA APTAMERS; ABSTRACT; APTAMERS AS A CLASS OF MOLECULAR RECOGNITION ELEMENTS; HD1, A KEY DNA APTAMER TO THROMBIN

ALTERING THE SEQUENCE OF HD1Altering Guanines of G-Quartets; Altering the TGT-Loop; Altering TT-Loops; ALTERING THE CONFORMATION OF HD1 WITH CATIONS; MODULATING THE ACTIVITY OF THROMBIN APTAMER WITH ADDITIONAL DUPLEX ELEMENT; MODULATING THE ACTIVITY OF THROMBIN APTAMER WITH ADDITIONAL G-QUADRUPLEX ELEMENT; SELECTIVITY OF THROMBIN APTAMERS TOWARD THROMBIN-RELATED PROTEINS; APTAMERS TO HEMAGGLUTININ WITH I-MOTIFS; CONCLUSION; REFERENCES; BIOGRAPHICAL SKETCH; Chapter 3: DOUBLE HELICES FORMING UNUSUAL BINDING MOTIFS: THE CASE OF THE NATURAL ALKALOIDS COPTISINE AND CHELERYTHRINE; ABSTRACT

INTRODUCTIONMATERIAL AND METHODS; Crystallization Experiments; X-ray Diffraction Analyses; QM and QM/MM Calculations; RESULTS; DISCUSSION; CONCLUSION; FUNDING; REFERENCES; BIOGRAPHICAL SKETCH; Chapter 4: G-QUADRUPLEX-INDUCED FLUORESCENCE SIGNAL; ABSTRACT; INTRODUCTION; Fluorescent Probes for G-Quadruplexes; NONCOVALENT PROBES FOR G-QUADRUPLEXES; Fluorescent Intercalator Displacement; FRET; Small Molecules; Cyanine Dyes; V-Shaped Planer Molecule; Metal Complexes; Thioflavin T; Triphenylmethane Dyes; Carbazole Derivatives; Aggregation-Induced Emission; Porphyrin Derivatives

COVALENT PROBES FOR G-QUADRUPLEXESCONCLUSION AND PERSPECTIVES; ACKNOWLEDGMENTS; REFERENCES; Chapter 5: G-QUADRUPLEX INTERACTING SMALL MOLECULES AND THEIR BIOLOGICAL IIMPLICATIONS; ABSTRACT; INTRODUCTION; STUDYING G-QUADRUPLEX BIOLOGY WITH SMALL MOLECULES; TELOMERE MAINTENANCE AND THE DNA DAMAGE RESPONSE; GENE TRANSCRIPTION AND TRANSLATION; GENOMIC CONTROL MECHANISMS; G-QUADRUPLEX INTERACTING SMALL MOLECULES AND CLINICAL APPLICATIONS; CONCLUSION; ACKNOWLEDGMENTS; REFERENCES; BIOGRAPHICAL SKETCH; Chapter 6: ASSAYS FOR TELOMERASE ACTIVITY TOWARD APPLICATIONS OF CANCER DIAGNOSIS ; ABSTRACT

1. INTRODUCTION

Notes:

Includes index.

Description based on print version record.

ISBN:

1-63485-550-7