Protein localization by fluorescence microscopy : a practical approach / edited by Victoria J. Allan.

Format:

Book

Contributor:

Allan, Victoria J., editor.

Series:

Practical approach series ; 218.

Oxford scholarship online.

Practical approach series ; 218

The practical approach series ; 218.

Oxford scholarship online

Language:

English

Subjects (All):

Proteins--Analysis.

Proteins.

Fluorescence microscopy.

Green fluorescent protein.

Immunofluorescence.

Physical Description:

1 online resource (xxiv, 231 p. ) ill. ;

Edition:

1st ed.

Place of Publication:

Oxford : Oxford University Press, 2023.

Language Note:

English

Summary:

An ever-increasing number of genes have been sequenced but are of unknown function. The ability to determine the location of gene products within the cell is a vital step towards understanding them. This guide provides practical advice.

Contents:

1. Basic immunofluorescence / Victoria J. Allan

2. Immunofluorescent labelling of sections / Alison J. North and J. Victor Small

3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld and Elisa M. Stone

4. Instruments for fluorescence imaging / W.B. Amos

5. Fluorescence microscopy of living vertebrate cells / Rainer Pepperkok and David Shima

6. Visualizing fluorescence in Drosophila

optimal detection in thick specimens / Ilan Davis

7. Green fluorescent protein in plants / Chris Hawes, Petra Boevink and Ian Moore

8. Fluorescence microscopy in yeast / Iain M. Hagan and Kathryn R. Ayscough

App. A1. Excitation and emission maxima of commonly used fluorophores

App. A2. Commercial sources of antibodies

App. A3. Optical units

App. A4. List of Suppliers.

Notes:

Previously issued in print: 1999.

Includes bibliographical references and index.

Derived record based on print version record and publisher information.

ISBN:

1-383-04963-7

9786610375684

1-280-37568-X

0-19-156591-1

0-585-48339-6

OCLC:

1027205972