Protein bioseparation using ultrafiltration : theory, applications and new developments / Raja Ghosh.

Format:

Book

Author/Creator:

Ghosh, Raja.

Language:

English

Subjects (All):

Proteins--Biotechnology.

Proteins.

Proteins--Separation.

Ultrafiltration.

Physical Description:

1 online resource (188 p.)

Place of Publication:

London : Imperial College Press, c2003.

Language Note:

English

Summary:

Ultrafiltration is a pressure-driven, membrane-based separation process, which is used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications account for nearly 40% of the current total usage of ultrafiltration membranes. Protein bioseparation is an important component of this application segment. Ultrafiltration is used for protein concentration, desalting, clarification and fractionation (i.e. protein-protein separation).

Contents:

Preface; Acknowledgements; Contents; List of Tables; List of Figures; 1 Protein Bioseparation: An Overview; 1.1 Introduction; 1.2 Proteins; 1.3 Protein products; 1.4 The requirement for protein bioseparation; 1.5 Economic aspects of protein bioseparation; 1.6 Protein bioseparation methods; 1.6.1 Cell disruption; 1.6.2 Precipitation; 1.6.3 Centrifugation; 1.6.4 Ultracentrifugation; 1.6.5 Column chromatography; 1.6.6 Electrophoresis; 1.6.7 Membrane chromatography; 1.6.8 Microfiltration; References; 2 Ultrafiltration: An Overview; 2.1 Introduction; 2.2 Applications of ultrafiltration

2.3 Advantages of ultrafiltrationReferences; 3 Membranes; 3.1 Introduction; 3.2 Membrane material and chemistry; 3.3 Membrane structure and morphology; 3.4 Membrane preparation; 3.5 Driving force in membrane separation processes; 3.6 Membrane characterisation; References; 4 Membrane Module and Operation; 4.1 Membrane elements and modules; 4.2 Mode of operation; References; 5 Membrane Fouling; 5.1 Introduction; 5.2 Fouling mechanisms; 5.2.1 External fouling; 5.2.1.1 Diffusional transport to membrane; 5.2.1.2 Bulk convection and sieving; 5.2.2 Internal fouling; 5.2.2.1 Direct interception

5.2.2.2 Inertial impaction5.2.2.3 Diffusional transport to pore walls; 5.3 Chemistry of adsorption; 5.4 Initial and long-term fouling; 5.5 Effect of concentration polarisation on fouling; 5.6 Effect of permeate flux on fouling, and the critical flux concept; 5.7 Effect of physicochemical parameters on fouling; References; 6 Permeate Flux in Ultrafiltration; 6.1 Permeate flux; 6.2 Enhancement of permeate flux; 6.3 Fouling control; References; 7 Protein Transmission Through Ultrafiltration Membranes; 7.1 Protein size; 7.2 Protein transmission; References

8 Selectivity of Protein Fractionation in Ultrafiltration8.1 Selectivity; 8.2 Enhancement of selectivity; References; 9 Protein Concentration; 9.1 Introduction; 9.2 Solute retention; 9.3 Permeate flux and fouling in concentration processes; 9.4 Modules; 9.5 Mode of operation; 9.6 Applications; References; 10 Diafiltration of Protein Solutions; 10.1 Introduction; 10.2 Permeate flux and fouling in diafiltration; 10.3 Modules; 10.4 Mode of operation; 10.5 Applications; References; 11 Protein Clarification; 11.1 Introduction; 11.2 Protein transmission

11.3 Permeate flux and fouling in protein clarification11.4 Particle transmission through UF membrane; 11.5 Modules; 11.6 Mode of operation; 11.7 Applications; References; 12 Protein Fractionation; 12.1 Introduction; 12.2 Challenges facing ultrafiltration based protein fractionation; 12.2.1 Broad pore size distribution; 12.2.2 Concentration polarisation; 12.2.3 Fouling/adsorption; 12.2.4 Protein-protein interactions; 12.2.5 Effects of physicochemical and process parameters on transmission; 12.3 Selectivity enhancement; 12.3.1 Proper membrane selection and membrane surface modification

12.3.2 pH and salt concentration optimisation

Notes:

Description based upon print version of record.

Includes bibliographical references.

ISBN:

9786611866785

9781281866783

1281866784

9781860949388

186094938X