Biological specimen preparation for transmission electron microscopy / Audrey M. Glauert, Peter R. Lewis.

Format:

Book

Author/Creator:

Glauert, Audrey M., author.

Lewis, P. R., author.

Series:

Practical methods in electron microscopy ; v. 17.

Practical methods in electron microscopy, 1353-6494 ; volume 17

Princeton legacy library

Language:

English

Subjects (All):

Mounting of microscope specimens.

Transmission electron microscopy--Technique.

Transmission electron microscopy.

Physical Description:

1 online resource (349 p.)

Edition:

Core Textbook

Place of Publication:

Princeton, New Jersey : Princeton University Press, [1998]

Language Note:

English

Summary:

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, EditorOriginally published in 1999.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Contents:

Frontmatter

Editor's preface to the series

Authors' preface and acknowledgements

Contents

1. An introduction to fixation and embedding procedures and their safe use in the laboratory

2. Fixatives

3. Fixation methods

4. Dehydration methods

5. Embedding methods

6. Embedding in epoxy resins

7. Embedding in acrylic resins

8. The Lowicryl resins and embedding at low temperatures

9. Other embedding media

10. Processing schedules

Appendix: List of suppliers

Subject index

Notes:

Description based upon print version of record.

Description based on print version record.

Includes bibliographical references and index.

ISBN:

9780691630120

0691630127

9780691600154

0691600155

9781400865024

1400865026

9780691009001

0691009007

OCLC:

884012588