Authentication of Chinese medicinal materials by DNA technology / [edited by] P.C. Shaw, J. Wang, P.P.-H. But.

Format:

Book

Contributor:

Chinese University of Hong Kong. Chung Chi College.

Shaw, P. C.

Wang, J.

But, Paul P. H.

Language:

English

Subjects (All):

Materia medica--China.

Materia medica.

DNA fingerprinting.

Medicine, Chinese.

Physical Description:

1 online resource (242 p.)

Place of Publication:

Singapore ; River Edge, NJ : World Scientific, c2002.

Language Note:

English

Summary:

Traditional Chinese medicine (TCM) has been used for centuries in the Orient to relieve or treat diseases. Its efficacy is now attracting worldwide attention. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. To ensure consistency of quality and avoid misuse, reliable programs are urgently needed for authentication. Proper authentication also assists in the detection and conservation of endangered species. DNA molecules offer a more definitive means of authentication, as genetic composition is unique for each taxon and is less affected by age, physiological conditions and environmental factors. This book is the first of its kind to introduce and evaluate the molecular techniques that have been developed to authenticate Chinese medicinal materials. The contents of the book are divided into four parts. The first part reviews the current status of molecular authentication of Chinese medicinal materials. The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM. The last part provides an overview of this field.

Contents:

1. Molecular markers in Chinese medicinal materials / Pang-Chui Shaw ... [et al.]

2. Methodology and equipment for general molecular techniques / Forrest Chung-Fai Yau and Fai-Ngor Ngan

3. Authentication of Chinese medicinal materials by polymerase chain reaction using arbitrarily chosen primers / Hui Cao ... [et al.]

4. Authentication of the components from a mixture of herbal materials / Kur-Ta Cheng

5. Authentication of Chinese medicinal materials by sequence characterised amplified region / Jun Wang ... [et al.]

6. Differentiation of panax ginseng from P. quinquefolius by direct amplification of length polymorphisms / Wai-Yan Ha ... [et al.]

7. Differentiation of panax ginseng from P. quinquefolius by amplified fragment length polymorphism / Wai-Yan Ha ... [et al.]

8. Authentication of animal medicinal materials by DNA molecular markers / Yiquan Wang and Kaiya Zhou

9. Authentication of medicinal dendrobium species by the internal transcribed spacer of ribosomal DNA / David Tai-Wai Lau, Pang-Chui Shaw and Paul Pui-Hay But

10. Authentication by DNA sequencing of 5s rRNA and trnK genes / Hajime Mizukami

11. Authentication based on 18S ribosomal RNA gene and matK gene sequences / Katsuko Komatsu

12. Outlook / Pang-Chui Shaw ... [et al.].

Notes:

"This volume is published in conjunction with the celebration of the 50th Anniversary of Chung Chi College, the Chinese University of Hong Kong."

Includes bibliographical references and index.

ISBN:

9786611908904

9781281908902

1281908908

9789812706591

9812706593

OCLC:

849916852