PCR applications : protocols for functional genomics / edited by Michael A. Innis, David H. Gelfand, John J. Sninsky.

Format:

Book

Contributor:

Sninsky, John J.

Innis, Michael A.

Gelfand, David H.

Language:

English

Subjects (All):

Polymerase chain reaction.

Gene amplification.

Polymerase chain reaction--Laboratory manuals.

Gene amplification--Laboratory manuals.

Genomics--Laboratory manuals.

Genomics.

Physical Description:

1 online resource (589 p.)

Place of Publication:

San Diego : Academic Press, c1999.

Summary:

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery

Contents:

Front Cover; PCR Applications: Protocols for Functional Genomics; Copyright Page; CONTENTS; Contributors; Preface; Part One: KEY CONCEPTS FOR PCR; Chapter 1. Optimization of PCR: Conversations between Michael and David; Chapter 2. The Convergence of PCR, Computers, and the Human Genome Project: Past, Present, and Future; Chapter 3. Thermostable DNA Polymerases: An Update; Chapter 4. Musings on Microbial Genomes; Chapter 5. Statistical Refinement of Primer Design Parameters; Chapter 6. Multiplex PCR: Optimization Guidelines

Chapter 7. The Use of Immobilized Mismatch Binding Protein for the Optimization of PCR FidelityChapter 8. A New Generation of PCR Instruments and Nucleic Acid Concentration Systems; Chapter 9. Sequencing PCR Products; Chapter 10. Recent Advances in High-Temperature Reverse Transcription and PCR; Chapter 11. Viral Genotyping by a Quantitative Point Mutation Assay: Application to HIV-1 Drug Resistance; Chapter 12. In Situ PCR; Part Two: QUANTITATIVE PCR; Chapter 13. Standards for PCR Assays; Chapter 14. Rapid Thermal Cycling and PCR Kinetics

Chapter 15. Kinetics of Competitive Reverse Transcriptase-PCRChapter 16. Kinetic PCR Analysis Using a CCD Camera and without Using Oligonucleotide Probes; Chapter 17. Quantification of Telomerase Activity Using Telomeric Repeat Amplification Protocol; Part Three: GENE DISCOVERY; Chapter 18. Differential Display; Chapter 19. Single-Cell cDNA Libraries; Chapter 20. Whole Cell Assays; Chapter 21. Screening Differentially Displayed PCR Products by Single-Strand Conformation Polymorphism Gels; Chapter 22. Microsatellite Protocols; Chapter 23. Real-Time Quantitative PCR: Uses in Discovery Research

Chapter 24. Homology Cloning: A Molecular Taxonomy of the ArchaeaChapter 25. Cloning Mammalian Homologs of Drosophila Genes; Chapter 26. Cloning Human Homologs of Yeast Genes; Part Four: GENOMICS AND EXPRESSION PROFILING; Chapter 27. Cellular Transcriptome Analysis Using a Kinetic PCR Assay; Chapter 28. Parallel Analysis with Biological Chips; Chapter 29. High-Density cDNA Grids for Hybridization Fingerprinting Experiments; Chapter 30. Comparative Genomic Hybridization; Chapter 31. Genetic Footprinting and Functional Maps of the Yeast Genome

Chapter 32. Molecular Analysis of Microdissected Tissue: Laser Capture MicrodissectionChapter 33. Amplified Fragment Length Polymorphism: Studies on Plant Development; Chapter 34. A Fluorescent, Multiplex Solid-Phase Minisequencing Method for Genotyping Cytochrome P450 Genes; Chapter 35. The Cleavase I Enzyme for Mutation and Polymorphism Scanning; Index; Color Plate Section

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-76378-0

9786611763787

0-08-091963-4

OCLC:

476220668