Gene expression systems : using nature for the art of expression / edited by Joseph M. Fernandez and James P. Hoeffler.

Format:

Book

Contributor:

Fernandez, Joseph M.

Hoeffler, James P.

Language:

English

Subjects (All):

Gene expression.

Prokaryotes--Genetics.

Prokaryotes.

Insects--Genetics.

Insects.

Transgenic animals--Genetics.

Transgenic animals.

Eukaryotic cells.

Physical Description:

1 online resource (493 p.)

Place of Publication:

San Diego : Academic Press, c1999.

Language Note:

English

Summary:

Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression.Key Features* Provides detailed information on expression systems* Covers a variety of promoters and

Contents:

Front Cover; Gene Expression Systems: Using Nature for the Art of Expression; Copyright Page; Contents; Contributors; Introduction; The Art of Expression; Purpose of This Book; Selecting a Suitable Expression System: Considerations; Genomics and the Future of Protein Expression Systems; SECTION I: PROKARYOTIC EXPRESSION SYSTEMS; Chapter 1. Gene Expression Systems Based on Bacteriophage T7 RNA Polymerase; Introduction; T7 RNAP in Its Natural Habitat; Biochemistry of T7 RNAP; T7-Based Gene Expression Systems; Conclusions; References; Chapter 2. Expression Vectors Employing the trc Promoter

IntroductionVectors for trc-Driven Expression; Additional Hosts for trc-Driven Expression; Outlook for Further Improvements of Expression Vectors Employing the trc Promoter; References; Chapter 3. Bacillus Expression"" A Gram-Positive Model; Introduction; Bacilli Used as Industrial Production Organisms; Genetic Manipulation in Bacilli; Plasmid Vectors; Transcriptional Regulation; Conclusions; Appendix; References; Chapter 4. araB Expression System in Escherichia coli; Introduction; The araB Promoter and How It Works; Use of the ara System for Expression of Recombinant Products; Conclusions

ReferencesSECTION II: EUKARYOTIC EXPRESSION SYSTEMS; Chapter 5. Adenoviral Vectors for Protein Expression; Adenovirus Biology; Adaptation of Adenovirus as a Gene Transfer Vector; Construction of Adenovirus Expression Vectors; Applications of Adenoviral Vectors in Gene Expression; Considerations in the Use of Adenovirus Expression Vectors; Advantages and Disadvantages of Adenoviral Vectors; Future Directions; Conclusions; References; Chapter 6. Expression in the Methylotrophic Yeast Pichia pastoris; Introduction; Background Information; Construction of Expression Strains

Important Considerations in the Use of the Expression SystemConclusion; References; Chapter 7. Recombinant Protein Expression in Pichia methanolica; Background; DNA Transformation; Expression Vector and Identification of an Expression Strain; Gene Disruptions and Generation of a Protease-Deficient Strain; Fermentation; Evaluation of Protein Expression; Expression of the 65-kDa Isoform of Human Glutamate; Protein Secretion; Benefits and Liabilities of the P. methanolica Expression System; Summary; Appendix; References; Chapter 8. Cytomegalovirus Promoter for Expression in Mammalian Cells

IntroductionThe Cytomegalovirus Enhancer-Containing Promoter; Positive Regulation; Experimental Procedures; Effects Upstream of the Cytomegalovirus Enhancer; Effects Downstream of the Cytomegalovirus Promoter; Negative Regulation; Conclusions; References; Chapter 9. Inducible Mammalian Expression Systems; Background: Development of Inducible Expression Systems; Literature Review; Author's Experience; Comparing Inducible Mammalian Expression Systems Currently Available; Conclusions and Future Directions; References; Chapter 10. Protein Expression in Mammalian Cells Using Sindbis Virus

Introduction

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-05418-6

9786611054182

0-08-053235-7

OCLC:

476102967