Mass spectrometry for biotechnology / Gary Siuzdak.

Format:

Book

Author/Creator:

Siuzdak, Gary.

Language:

English

Subjects (All):

Mass spectrometry.

Proteins--Analysis.

Proteins.

Nucleic acids--Analysis.

Nucleic acids.

Physical Description:

1 online resource (179 p.)

Place of Publication:

San Diego : Academic Press, 1996.

Summary:

If you investigate biological systems and might use mass spectrometry in your research but need to know more about it, this book is for you. It introduces the fundamental concepts of mass spectrometry and how mass spectrometers work. It also presents recent advancements particularly interesting to bio-researchers in an easy-to-understand manner that does not require extensive background in chemistry, math, or physics.Key Features* Glossary of basic terms* Abundant illustrations* Examples of applications* Practical tips on using mass spectrometric techniques* Useful

Contents:

Front Cover; Mass Spectrometry for Biotechology; Copyright Page; Contents; Preface; Acknowledgments; Historical Tabulation of Mass Spectrometry Developments in Biochemical Analysis; Introduction; What Is a Mass Spectrometer and What Does It Do?; Chapter 1. Ion Sources and Sample Introduction; Sample Introduction; Ionization Techniques; Mechanisms of Ionization; Calibration; Vacuum in the Mass Spectrometer; Summary; Practice Questions; Other Books on Mass Spectrometry; Chapter 2. Mass Analyzers and Ion Detectors; Resolution; Mass Analyzers; Tandem Mass Spectrometry; Ion Detectors; Summary

Practice QuestionsReferences; Chapter 3. Basics of Biomolecule Mass Spectrometry; General Preparative Techniques (FFAST-MS); Salt Purification; Calculating Molecular Weight; Quantifying; Liquid Chromatography-Mass Spectrometry; Capillary Zone Electrophoresis-Mass Spectrometry (CE-MS); Chapter 4. Peptide and Protein Analysis; Perspective; Peptide and Protein Analysis by FAB; Peptide and Protein Analysis by MALDI; Peptide and Protein Analysis by Electrospray; Sequencing of Peptides and Proteins; Summary; References; Chapter 5. Carbohydrates, Oligonucleotides, and Small Molecules; Perspective

FAB AnalysisMALDI Analysis; Electrospray Ionization; Summary; References; Chapter 6. Specific Applications; Chemical Characterization of a Family of Brain Lipids with Sleep- Inducing Potential; Monitoring Antibody Catalysis; Protein Conformational Changes Using Deuterium Exchange and Electrospray Mass Spectrometry; Characterizing Natural and Unnatural Peptides; Protein and Oligonucleotide Sequencing by MALDI-MS; Mass Spectrometry and Viral Analysis; Terms and Definitions; Appendix; Index

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-02943-2

9786611029432

0-08-053584-4

OCLC:

476098788