Molecular characterization / edited by Shmuel Razin, Joseph G. Tully.

Format:

Book

Contributor:

Razin, Shmuel.

Tully, Joseph G.

Series:

Molecular and diagnostic procedures in mycoplasmology ; 1

Language:

English

Subjects (All):

Mycoplasma diseases--Diagnosis.

Mycoplasma diseases.

Mycoplasma diseases--Molecular aspects.

Physical Description:

1 online resource (505 p.)

Place of Publication:

San Diego : Academic Press, c1995.

Language Note:

English

Summary:

This book and its companion, Volume II, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Volume II deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures, with particular emphasis on the association of mycoplasmas with the activation of AIDS.Key Features* Cultivation and mor

Contents:

Front Cover; Molecular and Diagnostic Procedures in Mycoplasmology; Copyright Page; Contents; Contributors; Preface; Contents of Volume II; Molecular Properties of Mollicutes: A Synopsis; SECTION A: Cultivation and Morphology; Chapter A1. Introductory Remarks; Chapter A2. Culture Medium Formulation for Primary Isolation and Maintenance of Mollicutes; Chapter A3. Cultivation of Spiroplasmas in Undefined and Defined Media; Chapter A4. Insect Cell Culture Approaches in Cultivating Spiroplasmas; Chapter A5. Measurement of Mollicute Growth by ATP-Dependent Luminometry

Chapter A6. Intracellular Location of MycoplasmasChapter A7. Localization of Mycoplasmas in Tissues; Chapter A8. Localization of Antigens on Mycoplasma Cell Surface and Tip Structures; SECTION B: Genome Characterization and Genetics; Chapter B1. Introductory Remarks; Chapter B2. Isolation of Mycoplasma-like Organism DNA from Plant and Insect Hosts; Chapter B3. Mollicute Chromosome Size Determination and Characterization of Chromosomes from Uncultured Mollicutes; Chapter B4. Physical and Genetic Mapping; Chapter B5. Characterization of Virus Genomes and Extrachromosomal Elements

Chapter B6. Plasmid and Viral Vectors for Gene Cloning and Expression in Spiroplasma citriChapter B7. Artificial Transformation of Mollicutes via Polyethylene Glycol- and Electroporation-Mediated Methods; Chapter B8. DNA Methylation Analysis; Chapter B9. Identification and Characterization of Genome Rearrangement; Chapter B10. Expression of Mycoplasmal Genes in Escherichia coli; SECTION C: Membrane Characterization; Chapter C1. Introductory Remarks; Chapter C2. Posttranslational Modification of Membrane Proteins; Chapter C3. Variant Membrane Proteins; Chapter C4. Membrane Fusion

Chapter C5. Mycoplasma Membrane PotentialsChapter C6. Ion Flow and Cell Volume; SECTION D: Cell Metabolism; Chapter D1. Introductory Remarks; Chapter D2. Methods for Testing Metabolic Activities; Chapter D3. Rapid Microcalorimetric and Electroanalytical Measurements of Metabolic Activities; Chapter D4. Characterization of Heat Shock Proteins; Chapter D5. Nucleolytic Activities of Mycoplasmas; Chapter D6. Proteolytic Activities; Chapter D7. Phospholipase Activity in Mycoplasmas; SECTION. E: Taxonomy and Phylogeny; Chapter E1. Introductory Remarks

Chapter E2. Minimal Standards for Description of New Species of the Class MollicutesChapter E3. Ribosomal RNA Sequencing and Construction of Mycoplasma Phylogenies; Chapter E4. Restriction Endonuclease Analysis; Chapter E5. Southern Blot Analysis and Ribotyping; Chapter E6. Phylogenetic Classification of Plant Pathogenic Mycoplasma-like Organisms or Phytoplasmas; Chapter E7. Determination of Cholesterol and Polyoxyethylene Sorbitan Growth Requirements of Mollicutes; SECTION F: Pathogenicity; Chapter F1. Introductory Remarks

Chapter F2. Mycoplasma Adherence to Host Cells : Methods of Quantifying Adherence

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-05440-2

9786611054403

0-08-053678-6

OCLC:

476103369