mRNA formation and function / edited by Joel D. Richter.

Format:

Book

Contributor:

Richter, Joel D.

Language:

English

Subjects (All):

Messenger RNA--Research--Methodology.

Messenger RNA.

RNA.

Physical Description:

1 online resource (419 p.)

Place of Publication:

San Diego : Academic Press, c1997.

Language Note:

English

Summary:

mRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed.Key Features* Isolation and characterization of specific RNA binding proteins* RNA metabolism and associated regulatory proteins* RNA detection and localization* A genetic approach to RNA function

Contents:

Front Cover; mRNA Formation and Function; Copyright Page; Contents; Contributors; Preface; Chapter 1. The Tat-TAR RNP, a Master Switch That Regulates HIV-1 Gene Expression; I. Introduction; II. Methods; References; Chapter 2. HeLa Nuclear Extract: A Modified Protocol; I. Growth of the HeLa Cells; II. Extract Preparation; Reference; Chapter 3. Functional Analysis of Splicing Factors and Regulators; I. Introduction; II. Functional Analysis of General Splicing Factors; III. Analysis of Regulatory Mechanisms in Pre-mRNA Splicing; References

Chapter 4. Identification of Pre-mRNA Splicing Factors and Analysis of RNA-Protein InteractionI. Introduction; II. Pre-mRNA Splicing and the Role of the Polypyrimidine Tract; III. Analysis of RNA Binding Proteins by UV Cross-Linking; IV. Analysis of Protein-RNA Interaction by Fluorescence Spectroscopy; V. Analysis of Pre-mRNA Splicing Complexes and Identification of Associated Factors by UV Cross-Linking; VI. Identification of RNA Binding Proteins by a cDNA Expression Library Screen; References; Chapter 5. In Vitro Analysis of Mammalian Cell mRNA 3' Processing; I. Introduction

II. General Considerations for the Analysis of mRNA 3' Processing in VitroIII. Pre-mRNA Cleavage and Polyadenylation; IV. 3' Processing Complex Formation; V. 3' Processing Complex Stability; VI. Probing 3' Processing Factor-Pre-mRNA Interactions by UV Cross-Linking; VII. Application of in Vitro Selection to the Analysis of Sequences That Direct 3' Processing; VIII. Conclusions; References; Chapter 6. Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins; I. Introduction; II. Preparation of Extracts; III. Preparation of RNAs; IV. Identification of Protein-RNA Complexes

V. Determination of RNA Sequence Requirements for Protein BindingVI. Cloning cDNAs for RNA Binding Proteins by Northwestern Screening; References; Chapter 7. Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae; I. Introduction; II. Poly(A) Tail Analysis; III. Polyadenylation Site Mapping by RT-PCR; IV. Preparation of Yeast mRNA 3' End Processing Extracts; References; Chapter 8. Poly(A) Polymerase/Cap-Specific 2'-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and Protein-Ligand Interaction Assays; I. Background

II. Expression and Purification of the Poly(A) Polymerase/ 2'- O-MethyltransferaseIII. Uses of the Poly(A) Polymerase/2'-O-Methyltransferase; IV. Assays Developed to Examine Protein-Ligand Interactions; References; Chapter 9. A Genetic Approach to Mapping Coding Region Determinants o f mRNA Instability in Yeast; I. Introduction; II. Experimental Methodology; III. Experimental Results; IV. Discussion and Conclusions; References; Chapter 10. Identification of the Protein That Interacts with the 3' End of Histone mRNA; I. Introduction; II. Detecting Specific Protein-RNA Interactions

III. In Vitro Processing of Histone Pre-mRNA

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-11932-6

9786611119324

0-08-053704-9

OCLC:

476083587