The SV40 replicon model for analysis of anticancer drugs / Robert M. Snapka.

Format:

Book

Author/Creator:

Snapka, Robert M., 1947-

Series:

Biotechnology intelligence unit.

Biotechnology intelligence unit

Language:

English

Subjects (All):

Antineoplastic agents--Research--Methodology.

Antineoplastic agents.

DNA-drug interactions.

SV40 (Virus).

Viral genetics.

Physical Description:

1 online resource (205 p.)

Place of Publication:

San Diego, Calif. : Academic Press ; Austin, Tex. : R.G. Landes, c1996.

Language Note:

English

Summary:

This book provides the most up-to-date review of the simian virus 40 (SV40) minichromosome as a model for the mammalian chromosome in studies of DNA replication. It focuses on disruption of DNA replication by anticancer drugs and DNA-damaging agents. There is a strong emphasis on the unique advantages of SV40 as an experimental system for the analysis of these classes of anticancer drug mechanisms. The new high-resolution gel electrophoresis methods for the analysis of SV40 DNA replication are covered in detail to aid readers in designing and interpreting similar experiments.Key Featur

Contents:

Front Cover; The SV40 Replicon Model for Analysis of Anticancer Drugs; Copyright Page; Contents; Chapter 1. Papovavirus Models for Mammalian Replicons; Papovaviruses; The SV40 Genome; SV40 Chromatin; Chapter 2. Analysis of SV40 DNA Replication Intermediates; Extraction of Viral DNA and Chromatin; Radiolabeling of SV40 DNA; Sucrose Density Gradient Ultracentrifugation; Isopycnic Centrifugation; High-Resolution Electrophoresis; Electron Microscopy; Filter Assays; Chapter 3. Catastrophic Failure of DNA Replication Forks: Structural and Recombinational Pathways

Collision of DNA Replication Forks with Drug-Stabilized Topoisomerase I-DNA Strand Passing ComplexesDNA Polymerase Inhibition; SV40 Replication Fork Instability and Mammalian Replicons; Chapter 4. Topoisomerase II and Termination of DNA Replication; The Termination Problem; Catalytic Topoisomerase II Inhibitors and Topoisomerase II Poisons; Assays for Catenated Dimers; Chapter 5. DNA Damaging Agents; Reactive Aldehydes; Ultraviolet (UV) Radiation; DNA Alkylating Agents; Chapter 6. Drug Discovery and Evaluation with SV40; Topoisomerase I Poison Signature Pattern

Topoisomerase II Inhibition and PoisoningInhibition of Protein Synthesis; DNA Replication Fork Arrest; Protein-DNA Crosslinkers; DNA Alkylating Agents; Validation of Assay; Detection and Purification of Topoisomerase II Inhibitors Using the SV40 Assay; Chapter 7. SV40 In Vitro DNA Replication: A Potential Model For Studying Anticancer Drug Action; Viral Components Required for SV40 In Vitro DNA Replication; Mammalian Cell Proteins Required for the Efficient Replication of SV40 Origin Containing DNA In Vitro; A Multiprotein Form of DNA Polymerase Mediates Papovavirus DNA Replication In Vitro

Anticancer Drug Action on In Vitro SV40 DNA Replication Activity in Crude Cell ExtractsAnticancer Drug Action on In Vitro SV40 DNA Synthesis Mediated by a Multiprotein Form ofDNA Polymerase; Perspective; Chapter 8. SV40 Circular Oligomer Series: Normal and Abnormal DNA Replication Intermediates; The Order of SV40 Circular Oligomers; Normal and Aberrant Replication Intermediates of the SV40 Circular Oligomers; Index

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-01891-0

9786611018917

0-08-055018-5

OCLC:

476087184