Techniques in protein chemistry VIII / edited by Daniel R. Marshak.

Format:

Book

Contributor:

Marshak, Daniel R.

Protein Society. Meeting 1996 : San Jose, Calif.)

Series:

Techniques in Protein Chemistry

Language:

English

Subjects (All):

Proteins--Analysis--Congresses.

Proteins.

Peptides--Analysis--Congresses.

Peptides.

Nucleotide sequence--Congresses.

Nucleotide sequence.

Physical Description:

1 online resource (933 p.)

Other Title:

Techniques in protein chemistry 8

Place of Publication:

San Diego ; London : Academic Press, c1997.

Language Note:

English

Summary:

Praise for the Series:""The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team.""--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-top reference tool for protein chemists, the ten section sof the book are divided by subject area to show the reader which techniques are currently applied to particular problems in protein science. This approach reflects current trends in which specific instruments and methodologies are used in

Contents:

Front Cover; Techniques in Protein Chemistry VIII; Copyright Page; Contents; Foreword; Preface; Acknowledgments; Section I: Primary Structural Analysis; Chapter 1. Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection; Chapter 2. Enhancement of Concentration Limits of Detection in Capillary Electrophoresis: Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization

Chapter 3. Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS)Chapter 4. Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis; Chapter 5. Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques; Chapter 6. Comparison of the High Sensitivity and Standard Versions of Applied Biosystems ProciseTM 494 N-Terminal Protein Sequencers Using Various Sequencing Supports

Chapter 7. Evaluation of ABRF-96SEQ: A Sequence Assignment ExerciseChapter 8. Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol; Chapter 9. A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing; Chapter 10. Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study; Section II: Physical and Chemical Analysis

Chapter 11. Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors: Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI DetectorsChapter 12. Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection; Chapter 13. A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides

Chapter 14. Sample Preparation Using Synthetic Membranes for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass SpectrometryChapter 15. Use of LC/MS Peptide Mapping for Characterization of Isoforms in 15N-Labeled Recombinant Human Leptin; Chapter 16. Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests; Chapter 17. Detecting and Identifying Active Compounds from a Combinatorial Library Using lAsys and Electrospray Mass Spectrometry

Chapter 18. Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization

Notes:

"Selected from presentations at the Tenth Symposium of the Protein Society, held in San Jose, August 3-7, 1996"--P. xvii.

Includes bibliographical references and index.

ISBN:

1-281-05431-3

9786611054311

0-08-054228-X

OCLC:

476103638