Introduction to biophysical methods for protein and nucleic acid research / edited by Jay A. Glasel, Murray P. Deutscher.

Format:

Book

Contributor:

Glasel, Jay A.

Deutscher, Murray P.

Language:

English

Subjects (All):

Proteins--Research--Methodology.

Proteins.

Nucleic acids--Research--Methodology.

Nucleic acids.

Physical Description:

1 online resource (528 p.)

Place of Publication:

San Diego : Academic Press, c1995.

Language Note:

English

Summary:

The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment.Key Features* Modern Use of Mass Spectroscopy* NMR Spectroscopy* Molecular Modeling and Graphics* Macintosh and DOS/Windows

Contents:

Front Cover; Introduction to Biophysical Methods for Protein and Nucleic Acid Research; Copyright Page; Contents; Contributors; Preface; Chapter 1. Basic Physical Properties of Proteins and Nucleic Acids; Glossary; I. Introduction; II. A History of the Concept That Molecules Can Be Very Large; III. Basic Properties of Macromolecules; IV. Fourier Transforms; V. Summary; References; Chapter 2. Electrophoretic Methods; Glossary; Symbols; I. Introduction; II. Gel Electrophoresis; III. Isoelectric Focusing; IV. Immunoelectrophoresis; V. Blotting; VI. Pulsed-Field Gel Electrophoresis

VII. Capillary ElectrophoresisReferences; Chapter 3. Hydrodynamic Methods; Glossary; Symbols; I. Introduction; II. Analytical Ultracentrifugation; III. Sucrose Density Gradient Sedimentation; IV. Diffusion; V. Sedimentation-Diffusion Equilibrium; VI. Viscosity Measurements; VII. Resources; References; Chapter 4. Mass Spectrometry; Glossary; I. Introduction; II. Instrumentation; III. Combined Techniques; IV. Applications; V. Conclusions; References; Chapter 5. Electron Microscopy; Glossary; I. Introduction; II. Principles of Electron Optics; III. Design and Operation of Electron Microscopes

IV. Methods of Sample PreparationV. Applications of Electron Microscopy in Cell and Molecular Biology; References; Chapter 6. Optical and Vibrational Spectroscopic Methods; Glossary; Symbols; I. Introduction; II. Electronic Spectroscopy; III. Vibrational Spectroscopy; Appendix; References; Chapter 7. Nuclear Magnetic Resonance; Glossary; I. Introduction; II. Basic Principles of Nuclear Magnetic Resonance Spectroscopy; III. Experimental Methods for Structure Determination; IV. Nuclear Magnetic Resonance of Proteins; V. Nuclear Magnetic Resonance of Nucleic Acids; VI. Conclusion; References

Chapter 8. Diffraction MethodsGlossary; Symbols; I. Introduction; II. Crystals; III. Collecting Diffraction Data; IV. Calculating Electron Density and Patterson Maps; V. Obtaining Phases; VI. Building Models; VII. Refinement; VIII. Evaluating the Model; IX. Analyzing the Model; X. Neutron Diffraction; XI. Fiber Diffraction; XII. Databases; XIII. New Directions; References; Chapter 9. Computational Techniques in Macromolecular Structural Analysis; Glossary; Acronyms; I. Introduction; II. Sequence Homology; III. Secondary Structure Predictions of Proteins

IV. Computers and Graphical RepresentationsV. Calculation of Structural, Thermodynamic, and Catalytic Properties; References; Index; Computer Program Supplement; Instructions for Using the Disk Supplements; Color Plate Section

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-03294-8

9786611032944

0-08-053498-8

OCLC:

476109412