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Reading, Writing, and Perturbing Human Neural Cell Biology / Sarshan Rubintheran Pather.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Pather, Sarshan Rubintheran, author.
- Language:
- English
- Subjects (All):
- Cellular biology.
- Molecular biology.
- Neurosciences.
- Cell and Molecular Biology--Penn dissertations.
- Penn dissertations--Cell and Molecular Biology.
- Local Subjects:
- Cellular biology.
- Molecular biology.
- Neurosciences.
- Cell and Molecular Biology--Penn dissertations.
- Penn dissertations--Cell and Molecular Biology.
- Physical Description:
- 1 online resource (192 pages)
- Distribution:
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- Contained In:
- Dissertations Abstracts International 84-12B.
- Place of Publication:
- [Philadelphia, Pennsylvania] : University of Pennsylvania, 2022.
- Language Note:
- English
- Summary:
- Unraveling the complexity of human brain function demands integrated profiling, generation, and creative disruption of cell type-specific states to decode genotype-phenotype relationships. Here, I present a thesis tackling each of these areas. Profiling or "reading" of human brain tissue at the single cell level with technical innovations in transcriptomics provides deep molecular and cellular reference maps describing the landscape of human neural cell types. Generation or "writing" of synthetic human neural tissue using emerging human induced pluripotent stem cell differentiation technologies provides tractable cellular models to study human-specific neural cell biology. Disruption or "perturbing" of human neural tissue with pooled genome editing CRISPR screening systems enables scalable discovery of novel genotype-phenotype relationships. Continued work in these areas will enhance our understanding of how human neural phenotypes shift from healthy to diseased states and inform the development of new therapeutics and diagnostics to drive human neural phenotypes from diseased to healthy states.
- Notes:
- Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
- Advisors: Shalem, Ophir; Song, Hongjun; Committee members: Anderson, Stewart A.; Berger, Shelley L.; Wu, Hao; Shi, Junwei.
- Department: Cell and Molecular Biology.
- Ph.D. University of Pennsylvania 2023.
- Local Notes:
- School code: 0175
- ISBN:
- 9798379750848
- Access Restriction:
- Restricted for use by site license.
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