Rapid Cell Magnetisation Using Cationised Magnetoferritin / by Sara Correia Carreira.

Format:

Book

Author/Creator:

Correia Carreira, Sara., Author.

Series:

Springer Theses, Recognizing Outstanding Ph.D. Research, 2190-5053

Language:

English

Subjects (All):

Biophysics.

Stem cells.

Magnetism.

Magnetic materials.

Nanochemistry.

Biological and Medical Physics, Biophysics.

Stem Cells.

Magnetism, Magnetic Materials.

Local Subjects:

Biological and Medical Physics, Biophysics.

Stem Cells.

Magnetism, Magnetic Materials.

Nanochemistry.

Physical Description:

1 online resource (XVII, 166 p. 90 illus., 16 illus. in color.)

Edition:

1st ed. 2017.

Place of Publication:

Cham : Springer International Publishing : Imprint: Springer, 2017.

Summary:

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.

Contents:

Introduction

Materials and Methods

Characterising Magnetoferritin and Cationised Magnetoferritin

Stem Cell Labelling with Cationised Magnetoferritin

Toxicological Profiling of Cationised Magnetoferritin

Escherichia coli labelling with Cationised Magnetoferritin

Overall Conclusions and Future Opportunities.

Notes:

Includes bibliographical references at the end of each chapters and index.

ISBN:

3-319-60333-7