Imaging, Manipulation and Optogenetics in Zebrafish / by Itia Amandine Favre‐Bulle.

Format:

Book

Author/Creator:

Favre‐Bulle, Itia Amandine., Author.

Series:

Springer Theses, Recognizing Outstanding Ph.D. Research, 2190-5053

Language:

English

Subjects (All):

Lasers.

Photonics.

Neurobiology.

Biophysics.

Optics, Lasers, Photonics, Optical Devices.

Biological and Medical Physics, Biophysics.

Local Subjects:

Optics, Lasers, Photonics, Optical Devices.

Neurobiology.

Biological and Medical Physics, Biophysics.

Physical Description:

1 online resource (XVIII, 86 p. 49 illus., 23 illus. in color.)

Edition:

1st ed. 2018.

Place of Publication:

Cham : Springer International Publishing : Imprint: Springer, 2018.

Summary:

The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.

Contents:

Introduction

Light Scattering in Brain Tissue Using Monte Carlo Method

Scattering in Zebrafish Brain for Optogenetics

Optical Systems to Decode Brain Activity

Investigation of Optical Properties of Otoliths with Optical Trapping

Optical Manipulation of Otoliths in-vivo

Conclusion.

Notes:

Includes bibliographical references.

ISBN:

3-319-96250-7