X-Ray Diffraction Imaging of Biological Cells / by Masayoshi Nakasako.

Format:

Book

Author/Creator:

Nakasako., Author.

Contributor:

Masayoshi..

Series:

Springer Series in Optical Sciences, 0342-4111 ; 210

Language:

English

Subjects (All):

Lasers.

Photonics.

Materials science.

Proteins.

Crystallography.

Physical measurements.

Measurement.

Optics, Lasers, Photonics, Optical Devices.

Characterization and Evaluation of Materials.

Protein Structure.

Crystallography and Scattering Methods.

Measurement Science and Instrumentation.

Local Subjects:

Optics, Lasers, Photonics, Optical Devices.

Characterization and Evaluation of Materials.

Protein Structure.

Crystallography and Scattering Methods.

Measurement Science and Instrumentation.

Physical Description:

1 online resource (XX, 228 p. 96 illus., 89 illus. in color.)

Edition:

1版. 2018.

Place of Publication:

Tokyo : Springer Japan : Imprint: Springer, 2018.

Summary:

In this book, the author describes the development of the experimental diffraction setup and structural analysis of non-crystalline particles from material science and biology. Recent advances in X-ray free electron laser (XFEL)-coherent X-ray diffraction imaging (CXDI) experiments allow for the structural analysis of non-crystalline particles to a resolution of 7 nm, and to a resolution of 20 nm for biological materials. Now XFEL-CXDI marks the dawn of a new era in structural analys of non-crystalline particles with dimensions larger than 100 nm, which was quite impossible in the 20th century. To conduct CXDI experiments in both synchrotron and XFEL facilities, the author has developed apparatuses, named KOTOBUKI-1 and TAKASAGO-6 for cryogenic diffraction experiments on frozen-hydrated non-crystalline particles at around 66 K. At the synchrotron facility, cryogenic diffraction experiments dramatically reduce radiation damage of specimen particles and allow tomography CXDI experiments. In addition, in XFEL experiments, non-crystalline particles scattered on thin support membranes and flash-cooled can be used to efficiently increase the rate of XFEL pulses. The rate, which depends on the number density of scattered particles and the size of X-ray beams, is currently 20-90%, probably the world record in XFEL-CXDI experiments. The experiment setups and results are introduced in this book. The author has also developed software suitable for efficiently processing of diffraction patterns and retrieving electron density maps of specimen particles based on the diffraction theory used in CXDI.

Contents:

Introduction

X-ray diffraction

Theory of X-ray diffraction imaging

Diffraction apparatus for X-ray diffraction imaging

Specimen preparation for X-ray diffraction imaging experiments at cryogenic temperature

Processing of diffraction patterns obtained from X-ray diffraction imaging experiments using X-ray free electron laser pulses

Phase retrieval of diffraction patterns

Projection structures of biological cells and organelles

Three-dimensional structural analyses in cryogenic X-ray diffraction imaging

Prospects for the structural analysis of biological specimens by X-ray diffraction imaging.

Notes:

Description based on publisher supplied metadata and other sources.

ISBN:

4-431-56618-X

OCLC:

1030411764