CRISPR-Cas Methods [electronic resource] : Volume 2 / edited by M. Tofazzal Islam, Kutubuddin Ali Molla.

Format:

Book

Contributor:

Islam, M. Tofazzal, Editor.

Molla, Kutubuddin Ali., Editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks, 1949-2456

Language:

English

Subjects (All):

Genetics.

Genetics and Genomics.

Local Subjects:

Genetics and Genomics.

Physical Description:

XVII, 397 p. 118 illus., 106 illus. in color. online resource.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.”.

Contents:

A Complete Methodology for the Instruction of CRISPR-based Gene Editing Using a Simplified Cell-free Extract System with Genetic Readout in Bacteria

In silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing

Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants

CRISPR-dCas9 Based Targeted Manipulation of DNA Methylation in Plants

Rapid Assembly of Multiplex Natural CRISPR Arrays

Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants

Designing, Performing and Analysing CRISPR-Cas9-mediated Genome Editing Experiments in Leguminous Plant

Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9

Targeted Base Editing in Soybean using a CRISPR-Cas9 Cytidine Deaminase Fusion

Genome Editing in Potato Using a Hairy Root Transformation System

Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato

CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single and Multi-Target Vector Construction

Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9

CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants

CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells

Cell-Type-Specific CRISPR-Cas9 System with miRNAs

CRISPR-Cas9 gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-puro Vectors

An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA that Regulate CRISPR-Cas systems

Genetic Engineering of a Phage-based Delivery System for Endogenous III-A CRISPR-Cas System against Mycobacterium tuberculosis

CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages

CRISPR-Cas Genome Editing in the Cellulolytic Bacterium Clostridium thermocellum

Gene Targeting in Caenorhabditis elegans Using a Combination of Multiple sgRNAs and a Homologous Recombination-mediated Repair

Design of Repair Templates for CRISPR-Cas9-triggered Homologous Recombination in Caenorhabditis elegans.

Other Format:

Printed edition:

ISBN:

9781071616574