Human Embryonic and Induced Pluripotent Stem Cells [electronic resource] : Lineage-Specific Differentiation Protocols / edited by Kaiming Ye, Sha Jin.

Format:

Book

Contributor:

Ye, Kaiming, Editor.

Jin, Sha, Editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks, 1949-2456

Language:

English

Subjects (All):

Cytology.

Stem cells.

Cell Biology.

Stem Cell Biology.

Local Subjects:

Cell Biology.

Stem Cell Biology.

Physical Description:

XVI, 486 p. 88 illus., 48 illus. in color. online resource.

Edition:

1st ed. 2012.

Contained In:

Springer Nature eBook

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana, 2012.

System Details:

text file PDF

Summary:

Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world. The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies. Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.

Contents:

Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

Growth of Human Embryonic Stem Cells in Long-Term Hypoxia

Laboratory–Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein that Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells

Scale-Up of Single Cell-Inoculated Suspension Cultures of Human Embryonic Stem Cells

Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells

Expansion of Pluripotent Stem Cells in Defined Xeno-Free Culture System

Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells

Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2

Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage

Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells

Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes

Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles

Generation of iPS Cells from Human Skin Biopsy

Generation of Induced Pluripotent Stem Cells from Human Amnion Cells

In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells

Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells

Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endothelial Cells under Hypoxic Condition

Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture with Murine Stromal Cells

Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells

Adipogenic Differentiation of Human Induced Pluripotent Stem Cells

Chondrogenic Differentiation of hESC in Micromass Culture

Deriving Hepatic Endoderm from Pluripotent Stem Cells

Multistage Hepatic Differentiation from Human Induced Pluripotent Stem Cells

Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers

Generation of Lung Epithelial-Like Tissue from hESC by Air-Liquid Interface Culture

Direct Differentiation of Human Embryonic Stem Cells into Selective Neurons on Nanoscale Ridge/Groove Pattern Arrays

Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and MatrigelTM Gels

Single-Cell Transcript Profiling of Differentiating Embryonic Stem Cells

Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells.

Other Format:

Printed edition:

ISBN:

9781617792670