Sample Preparation Techniques for Soil, Plant, and Animal Samples [electronic resource] / edited by Miodrag Micic.

Format:

Book

Contributor:

Mićić, Miodrag, Editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks, 1949-2456

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

XXIII, 406 p. 96 illus., 65 illus. in color. online resource.

Edition:

1st ed. 2016.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2016.

System Details:

text file PDF

Summary:

The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation.

Contents:

Physical and Chemical Properties of Nucleic Acids

Sampling for Biostatistics

Sampling of Microbiological Samples

Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples

Magnetic beads based nucleic acids purification for molecular biology applications

Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications

Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples

High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and proteins extraction with FastPrep® Systems

Portable Systems for Sample Lysis and Homogenization

DNA and RNA extractions from mammalian samples

Sample preparation for multiplex PCR assays for food and agriculture applications

Nucleic acid sample preparation from stem cells

Nucleic Acids Sample Preparation from Teeth/Dental Remains

Nucleic Acids Sample Preparation for Food Traceability

Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis

Nucleic Acids Samples Preparation from Dairy Products and Milk

Extraction of DNA from Plant Tissue: Review and Protocols

DNA Extraction from Seeds

RNA extractions from difficult to prepare and high starch content seeds

Nucleic Acid and Protein Sample Preparation from Yeasts

Nucleic acids purification from soil and environmental sources

Extraction and Purification of Viral Nucleic Acids from Environmental Samples

Preparation of nucleic acids from marine samples: applications to microbial ecology research

Nucleic Acid Sample Preparation from Feces and Manure

Bead-beating sample preparation for nucleic acids isolation from fecal samples

Methods for extraction and purification of lignin and cellulose from plant tissues

Separation and purification of glycans out of glycoproteins

Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria.

Other Format:

Printed edition:

ISBN:

9781493931859