Advanced Protocols for Animal Transgenesis [electronic resource] : An ISTT Manual / edited by Shirley Pease, Thomas L. Saunders.

Format:

Book

Contributor:

Pease, Shirley, Editor.

Saunders, Thomas L., Editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks, 1949-2456

Language:

English

Subjects (All):

Genetics.

Biology—Technique.

Genetics and Genomics.

Experimental Organisms.

Local Subjects:

Genetics and Genomics.

Experimental Organisms.

Physical Description:

XV, 669 p. 210 illus., 65 illus. in color. online resource.

Edition:

1st ed. 2011.

Contained In:

Springer Nature eBook

Place of Publication:

Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2011.

System Details:

text file PDF

Summary:

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Contents:

Karen S. Canady: Patent and licensing issues in transgenic technology

Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?

Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression

Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications

Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks

Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection

Séverine Ménoret, Séverine Remy, et al : Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors

Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs

Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production

Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors

Aron Geurts, Lajos Mates, Darius Balciunas: Vertebrate Transgenesis by Transposition

Karen M. Chapman et al.: Rat Spermatogonial Stem Cell Mediated Gene Transfer

Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer

Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells

Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection

Marina Gertsenstein: Tetraploid Complementation Assay

Elizabeth Williams et al.: Combining ES cells with Embryos

Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines

Ping Li et al.: Rat Embryonic Stem Cell Derivation and Propagation

Han Li et al.: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts

Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology

Karen Brennan: Colony Management

B. Pintado and J Hourcade: Cryopreservation

Shirley Pease: Shipment of Mice and Embryos

J.M. Sztein, R.J. Kastenmayer, K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy

Jan Parker-Thornburg: Refinement, Reduction and Replacement.

Other Format:

Printed edition:

ISBN:

9783642207921