Hydrocarbon and Lipid Microbiology Protocols [electronic resource] : Synthetic and Systems Biology - Applications / edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales Fernández.

Format:

Book

Contributor:

McGenity, Terry J., Editor.

Timmis, Kenneth N., Editor.

Nogales Fernández, Balbina., Editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks, 1949-2456

Language:

English

Subjects (All):

Microbiology.

Biotechnology.

Chemical Bioengineering.

Local Subjects:

Microbiology.

Chemical Bioengineering.

Physical Description:

X, 137 p. 40 illus., 20 illus. in color. online resource.

Edition:

1st ed. 2016.

Contained In:

Springer Nature eBook

Place of Publication:

Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2016.

System Details:

text file PDF

Summary:

This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Contents:

Introduction

Designing bacteria to produce esters

Protocols for the production and analysis of isoprenoids in bacteria and yeast

Synthetic biology of hydrophobic polymer production

Methods for recombinant rhamnolipid production

Purification of peptide antimicrobials and thioether stabilized molecules produced in vivo by lantibiotic modification machineries

Engineering transcription factor based biosensors for the detection of intracellular products

Oxyfunctionalization of linear alkanes with a biosynthetic, self-sufficient, selective and soluble hydroxylase. .

Other Format:

Printed edition:

ISBN:

9783662491270