Basic Techniques in Biochemistry, Microbiology and Molecular Biology [electronic resource] : Principles and Techniques / by Aakanchha Jain, Richa Jain, Sourabh Jain.

Format:

Book

Author/Creator:

Jain, Aakanchha, Author.

Jain, Richa, Author.

Jain, Sourabh, Author.

Contributor:

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2456

Springer Protocols Handbooks, 1949-2456

Language:

English

Subjects (All):

Biotechnology.

Biology—Technique.

Pharmaceutical chemistry.

Biological Techniques.

Pharmaceutics.

Local Subjects:

Biotechnology.

Biological Techniques.

Pharmaceutics.

Physical Description:

XIV, 282 p. 50 illus., 39 illus. in color. online resource.

Edition:

1st ed. 2020.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.

Contents:

Chapter 1. BOD (Biochemical oxygen demand or biological oxygen demand) Incubator

Chapter 2. Laminar Air Flow/ Biosafety Cabinets

Chapter 3. Aseptic Hood

Chapter 4. Autoclave

Chapter 5. Hot Air Oven

Chapter 6. Deep Freezer (-20 0C) (Low Temperature Cabinet)

Chapter 7. Refrigerator

Chapter 8. Compound Microscope

Chapter 9. Digital Colony Counter

Chapter 10. Digital Turbidity Meter

Chapter 11. Digital Nephel Ometer

Chapter 12. Digital Colorimeter

Chapter 13. Digital UV / Visible Spectrophotometer

Chapter 14. Polymerase Chain Reaction

Chapter 15. ELISA Reader

Chapter 16. Sonicator

Chapter 17. Enzyme Assay: Qualitative and Quantitative (Protease, Amylase, Penicillinase, Pectinolytic Enzyme, Chitinase, Keratinase)

Chapter 18. Antimicrobial Sensitivity Assay

Chapter 19. Extraction of DNA from plant cells

Chapter 20. Extraction of DNA from Animal cells

Chapter 21. Extraction of DNA and RNA from microorganism

Chapter 22. Estimation of DNA by Diphenylamine Reaction

Chapter 23. Estimation of RNA using Orcinol Method

Chapter 24. Determination of Melting Temperature of DNA

Chapter 25. Quantitative Estimation of DNA AND RNA

Chapter 26. Purification and Bioassay of Interleukin 1 and 2 (IL 1 AND IL 2)

Chapter 27. Object: Quantitative Determination of TNF Alpha and Interleukin 6 in the given sample

Chapter 28. Sterilization of Glassware, Preparation of Media and its Sterilization

Chapter 29. Sub Culturing of Bacteria, Fungi and Actinomycetes

Chapter 30. Preservation of Microorganisms: Stabs, Slants, Lyophilization, Cryopreservation

Chapter 31. Staining Methods: Simple, Negative, Gram Staining, Acid-Fast Staining, Cell Wall, Capsule, Flagella, Nuclear material, Cytoplasm, Filamentous Fungi, Mycoplasma

Chapter 32. Isolation of Coprophilous Fungi (By Moist Chamber Method)

Chapter 33. Isolation of Microorganisms from air

Chapter 34. Motility Testing: Hanging Drop Method and Stab

Chapter 35. Sterility Testing of Pharmaceuticals

Chapter 36. Physical, Chemical and Bacteriological Analysis of Water. (a) Physical and Chemical Analysis of Water (b) Bacteriological Analysis of Water

Chapter 37. To Perform Biochemical Identification of Microorganism: Imvic, Oxidative Fermentation, Carbohydrate Source Utilization

Chapter 38. To Perform Identification of Microorganism by Urease Test

Chapter 39. Immobilization of Enzymes and Microorganisms

Chapter 40. Qualitative Systematic Analysis of Carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and Starch)

Chapter 41. Protein Analysis in Food

Chapter 42. Identification Tests for Proteins (Casein and Albumin)

Chapter 43. Quantitative Analysis of Reducing Sugars by 3, 5-Dinitrosalicylic Acid (Dnsa Method)

Chapter 44. Quantitative Analysis of Proteins by Various Methods Including Biuret

Chapter 45. Qualitative Anaylsis of Urine for Abnormal Constituents

Chapter 46. Determination of Blood Creatinine

Chapter 47. Determination of Blood Sugar

Chapter 48. To Perform Oral Glucose Tolerance Test

Chapter 49. Determination of Serum Total Cholesterol

Chapter 50. Preparation of Buffer Solution and Measurement of PH

Chapter 51. Study of Enzymatic Hydrolysis of Starch

Chapter 52. Determination of Salivary Amylase Activity

Chapter 53. Study the Effect of Temperature on Salivary Amylase Activity

Chapter 54. Study the Effect of Substrate Concentration on Salivary Amylase Activity

Chapter 55. Analysis of Butter

Chapter 56. Analysis of Milk

Chapter 57. Tlc of Carbohydrates

Chapter 58. Tlc of Amino Acid

Chapter 59. Paper Chromatography of Amino Acid

Chapter 60. Paper Chromatography of Carbohydrates

Chapter 61. Fat Characterization

Chapter 62. Analysis of Bread.

Other Format:

Printed edition:

ISBN:

9781493998616