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The Biology of CD8 T Cell Exhaustion: Lessons from in vitro Models / Jennifer Ening Wu.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Wu, Jennifer Ening, author.
- Language:
- English
- Subjects (All):
- Immunology.
- Cellular biology.
- Immunology--Penn dissertations.
- Penn dissertations--Immunology.
- Local Subjects:
- Immunology.
- Cellular biology.
- Immunology--Penn dissertations.
- Penn dissertations--Immunology.
- Physical Description:
- 1 online resource (188 pages)
- Contained In:
- Dissertations Abstracts International 84-08B.
- Place of Publication:
- [Philadelphia, Pennsylvania] : University of Pennsylvania, 2022.
- Ann Arbor : ProQuest Dissertations & Theses, 2022
- Language Note:
- English
- Summary:
- Identifying novel molecular mechanisms of exhausted CD8 T cells (Tex) is a key goal of improving immunotherapy of cancer and other diseases. However, high-throughput interrogation of in vivo Tex can be costly and inefficient. In vitro models of Tex are easily customizable and quickly generate high cellular yield, offering an opportunity to perform CRISPR screening and other high-throughput assays. We established an in vitro model of chronic stimulation and benchmarked key phenotypic, functional, transcriptional, and epigenetic features against bona fide in vivo Tex. We leveraged this model of in vitro chronic stimulation in combination with pooled CRISPR screening to uncover transcriptional regulators of T cell exhaustion. This approach identified several transcription factors, including Bhlhe40. In vitro and in vivo validation defined a role for Bhlhe40 in regulating a central differentiation checkpoint between progenitor and intermediate subsets of Tex. By developing and benchmarking an in vitro model of Tex, we demonstrate the utility of mechanistically annotated in vitro models of Tex, in combination with high-throughput approaches, as a discovery pipeline to uncover novel Tex biology.
- Notes:
- Source: Dissertations Abstracts International, Volume: 84-08, Section: B.
- Advisors: Wherry, E. John; Committee members: Laufer, Terri M.; Hunter, Christopher A.; Henao-Mejia, Jorge; Beiting, Daniel P.; Vahedi, Golnaz.
- Department: Immunology.
- Ph.D. University of Pennsylvania 2022.
- Local Notes:
- School code: 0175
- ISBN:
- 9798374413519
- Access Restriction:
- Restricted for use by site license.
- This item is not available from ProQuest Dissertations & Theses.
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