Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose / Sören Starck.

Format:

Book

Author/Creator:

Starck, Sören, author.

Series:

Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes

Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes ; v.8

Language:

English

Subjects (All):

Hydrolysis.

Physical Description:

1 online resource (135 pages)

Edition:

1st ed.

Place of Publication:

Germany : Cuvillier Verlag, [2022]

Summary:

Cis, cis-muconic acid receives increasing interest to be produced from renewables. Catabolic microbial pathways can be tailored to accumulate cis, cis-muconic acid from a range of aromatic compounds. A renewable, sustainable and under-valued resource for aromatics is lignin. In this work, using hydrothermal conversion, lignin was depolymerized into hydrolysates with up to 615 mM aromatic monomer content. Catechol-rich hydrolysates were generated for bioconversion with the previously developed cis, cis-muconic acid producers P. putida MA-9 and C. glutamicum MA-2, whereas hydrolysates were guaiacol-rich for Amycolatopsis sp. MA-2. When grown with glucose as a co-substrate, C. glutamicum MA-2 yielded 2.6 g L?¹ (100 % yield) cis, cis-muconic acid from catechol. Towards an even more sustainable process, glucose was then replaced by hemicellulose, a non-food renewable. Hemicellulose, a co-constituent of lignin in lignocellulose, was hydrothermally converted into a mixture of C? and C? sugars. As hemicellulose was mainly converted into xylose (91 % yield), C. glutamicum MA-2 was engineered to utilize this pentose. Fed-batch bioconversion on a catechol-rich Kraft lignin hydrolysate as well as a hemicellulose hydrolysate using C. glutamicum MA-4 yielded 4 g L?¹ muconic acids. As the developed process was non-competitive to feed and food, it is a promising starting point for future application in bio-based industrial settings.

Contents:

Intro

Summary

Zusammenfassung

1 Introduction

1.1 General introduction

1.2 Objectives

2 Theoretical Background

2.1 Biomass as a resource

2.2 Depolymerization of biomass

2.3 Bioconversion of biomass-derived carbon

2.4 Cis,cis-muconic acid - an important platform chemical

3 Material and Methods

3.1 Lignin and hemicellulose

3.2 Hydrothermal lignin conversion

3.3 Hydrothermal lignin conversion at miniaturized scale

3.4 Hydrothermal hemicellulose conversion

3.5 Fractionation of depolymerized hemicellulose by distillation

3.6 Distillation of lignin hydrolysate

3.7 Concentration of lignin and hemicellulose hydrolysates

3.8 Decolorization of hemicellulose hydrolysates and removal oftoxic ingredients

3.9 Microorganisms and plasmids

3.10 Media

3.11 Cultivation of Pseudomonas putida

3.12 Cultivation of Corynebacterium glutamicum strains

3.13 Cultivation of Amycolatopsis sp.

3.14 Fed-batch production of cis,cis-muconic acid

3.15 Quantification of glucose and xylose

3.16 Quantification of mono- and disaccharides

3.17 Quantification of aromatics and muconic acid derivatives

3.18 Fingerprinting of aromatics in lignin hydrolysates

3.19 Quantification of organic acids and alcohols

3.20 Quantification of cell concentration

4 Results and Discussion

4.1 Microbial production of cis,cis-muconic acid from aromatics

4.2 Cascaded production of cis,cis-muconic acid from Kraft lignin

4.3 Impact of the lignin-type on depolymerization and bioconversionefficiency

4.4 Gram-scale production of cis,cis-muconic acid from repeatedfeeding of lignin hydrolysate

4.5 The use of hemicellulose-derived sugars as co-substrate enablesan entirely lignocellulose-based process

5 Conclusion and Outlook

6 Appendix

6.1 Abbreviations.

6.2 Cultivation of P. putida MA-9 on organosolv lignin hydrolysate

6.3 Aromatic monomers obtained from multiple runs of hydrothermal

6.4 Cultivation of C. glutamicum MA-3 on hemicellulose hydrolysatein a bioreactor with addition of lignin hydrolysate

6.5 Overview of results from hydrothermal conversion of lignin andsubsequent bioconversion to muconic acids

7 References.

Notes:

Description based on print version record.

Other Format:

Print version: Starck, Sören Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose

ISBN:

9783736965898