Practical approach to mammalian cell and organ culture / edited by Tapan Kumar Mukherjee, Parth Malik, and Srirupa Mukherjee.

Format:

Book

Contributor:

Mukherjee, Tapan Kumar, editor.

Malik, Parth., editor.

Mukherjee, Srirupa, editor.

Language:

English

Subjects (All):

Cell culture.

Organ culture.

Physical Description:

1 online resource (1286 pages)

Edition:

1st ed. 2023.

Place of Publication:

Singapore : Springer, [2023]

Summary:

This Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures.

Contents:

Mammalian Cell Culture: An Overview

Establishment of a Cell Culture Laboratory

Mammalian Cell Culture Laboratory: Equipment and Other Materials

Common Reagents and Medium for Mammalian Cell Culture

Microbial Contamination of Mammalian Cell Culture

Mammalian Cell Culture Types and Guidelines for Their Maintenance

Isolation and Purification of Various Mammalian Cells: Single Cell Isolation

Isolation and Primary Culture of Various Mammalian Cells

Primary Culture of Immunological Cells

Culture of Neuron and Glia Cells

Culture of Continuous Cell Lines

Stem Cell Culture and Its Applications

Mammalian Cell Culture in Three Dimensions: Basic Guidelines

Organ, Histotypic and Organotypic Culture, and Tissue Engineering

Large-Scale Culture of Mammalian Cells for Various Industrial Purposes

Experimental Mammalian Cell Culture-Based Assays

Mammalian Cells, Tissues and Organ Culture: Applications

Biosensors’ Utility in Mammalian Cell Culturing

Nanomaterials: Compatibility Towards Biological Interactions

Review on IPR and Technological Advancements in Nanotechnology for Nanomedicine

Emerging Drug Delivery Potential of Gold and Silver Nanoparticles to Lung and Breast Cancers

Mammalian Cells: Reliability as Model System in the Ecotoxicological Evaluation of Environmental Stressors

Bioimaging: Usefulness in Modern Day Research

Troubleshooting of Mammalian Cell Culture.

Notes:

Includes bibliographical references and index.

Description based on print version record.

ISBN:

981-19-1731-0

OCLC:

1371023462