Material-Based Mechanobiology / edited by Jun Nakanishi and Koichiro Uto.

Format:

Book

Contributor:

Nakanishi, Jun, editor.

Uto, Koichiro, editor.

Series:

Biomaterials science series.

Biomaterials science series

Language:

English

Subjects (All):

Cells--Mechanical properties.

Cells.

Materials science.

Physical Description:

1 online resource (365 pages)

Edition:

First edition.

Place of Publication:

London, England : The Royal Society of Chemistry, [2022]

Summary:

This book focuses on recent progress in mechanobiology from the materials science perspective.

Contents:

Cover

Preface

Contents

Chapter 1 An Introduction to Material-based Mechanobiology

1.1 Overview of Material-based Mechanobiology

1.2 Historical Background of Mechanobiology

1.2.1 Before the Dawn of Molecular Mechanobiology

1.2.2 Dawn of Molecular Mechanobiology: Mechanosensory Molecules and Molecular Assemblies

1.3 Material-based Mechanobiology

1.3.1 Material-based Mechanobiology: Form

1.3.2 Material-based Mechanobiology: Matrix Mechanics

1.3.3 Material-based Mechanobiology: Force Detection

1.4 Future Directions of Material-based Mechanobiology

References

Chapter 2 On the Molecular Basis of Cellular Mechanobiology

2.1 Mechanosensing at the Cell-Matrix Interface

2.1.1 Origin of the Mechanical Signals

2.1.2 Cellular Membrane as Prime Mechanosensing Structure

2.1.3 Mechanosensitive Ion Channels

2.1.4 Focal Adhesions (FAs)

2.2 Mechanotransduction at the Cytoskeleton

2.2.1 Cytoskeleton Network

2.2.2 Regulation of Cytoskeleton Intracellular Tension: Rho/ROCK Pathway

2.3 Transferring Mechanical Cues to the Nucleus

2.3.1 Nucleus as a Mechanosensor

2.3.2 Linker of Nucleoskeleton and Cytoskeleton (LINC) Complex

2.3.3 Nucleoskeleton and Transcriptional Activity Control

2.3.4 Nucleo-cytoskeleton Coupling in Cellular Adaptation to Mechanical Stress

2.3.5 Shuttling Mechanosensors and Mechanosensitive Transcription Factors

2.4 Conclusions and Perspectives

Acknowledgements

Chapter 3 Mechanotransduction at the Cell Surface and Methods to Study Receptor Forces

3.1 Introduction

3.2 Mechanotransduction at the Cell Surface

3.2.1 Mechanotransduction Through Integrins

3.2.2 Mechanotransduction Through T Cell Receptor

3.3 Methods to Study Receptor Forces

3.3.1 DNA Mechanics

3.3.2 DNA-based Molecular Force Sensing.

3.4 Conclusion and Future Perspectives

Chapter 4 Measurement and Manipulation of Cellular Forces Using Silicone Elastomers

4.1 Introduction

4.2 Mechanical Measurement Using Silicone Elastomers

4.2.1 Measurement of Force at Cell-ECM Adhesion

4.2.2 Measurement of Force at Cell-Cell Adhesion

4.2.3 Cell Stiffness Measurement Using Microfluidics

4.3 Mechanical Manipulation by Stretching Silicone Elastomer Membranes

4.3.1 Distinct Strain Types

4.3.2 Sustained or Cyclic

4.4 Combination of Mechanical Manipulation and Measurement

4.5 Concluding Remarks

Chapter 5 Geometric Cues for Directing Cell Fate

5.1 Introduction

5.2 Microengineering Techniques to Control the Shape of Cells and Tissue

5.2.1 Photolithography

5.2.2 Soft Lithography

5.2.3 Inkjet Cell Printing

5.2.4 Optical Tweezers and Optoelectronic Tweezers

5.2.5 Laser-based Cell Patterning

5.2.6 Dielectrophoresis

5.2.7 Other Techniques

5.3 Controlling Tissue Geometry in 3D

5.4 Deconstructing Biological Behavior Through Micropatterning

5.4.1 Teasing out Mechanotransduction During Stem Cell Differentiation

5.4.2 Controlling Shape and Interfacial Topography in Cancer Cultures

5.5 Conclusion and Future Perspectives

Abbreviations

Chapter 6 Dynamic Interfaces for Mechanobiological Studies

6.1 Introduction

6.2 Switchable Interfaces

6.2.1 Switchable Interfaces for the Spatiotemporal Control of Cell Adhesion

6.2.2 Switchable Interfaces at Subcellular and Molecular Resolutions

6.2.3 Switchable Interfaces for Exploring Dynamic Mechanobiology

6.3 Compliant Interfaces

6.3.1 Dissipative Interfaces

6.3.2 Liquid-Liquid Interfaces

6.3.3 Force-actuating Materials

6.4 Conclusions and Future Perspectives

Acknowledgements.

References

Chapter 7 Micro- and Nanopatterned Substrates for Studies on the Mechanobiology of Cell-Matrix Adhesions

7.1 Chemomechanical Coupling at the Cell-Extracellular Matrix Interface

7.2 Spatial Control of Cell-Extracellular Matrix Adhesion by Surface Patterning

7.2.1 Micropatterning of Matrix Proteins to Direct Cell Shape and Architecture

7.2.2 Nanopatterning of Matrix Ligands for the Control of Receptor Clustering

7.3 Surface Patterning for Cell-generated Force Measurements

7.3.1 Micro- and Nanopatterning of Soft Materials to Probe Cell Rigidity Sensing and Measure Cellular Traction Forces

7.3.2 Nanopatterning and Measurements of Molecular Forces at Cell-Matrix Adhesion Sites

7.4 Outlook

Chapter 8 Role of Topographic Cues in Engineering the Muscle Niche

8.1 Introduction

8.1.1 Historical Background

8.1.2 Structure-Function Relationship in Muscle Biology

8.1.3 Importance of Substrate Patterning for Muscle Tissue Engineering and Its Applications

8.2 Fabricating Substrate Topographies for Use in Cell Culture

8.2.1 Template-assisted Fabrication

8.2.2 Template-free Fabrication

8.3 Use of Patterned Surfaces to Enhance Muscle Cells in Culture

8.3.1 Patterned Cardiomyocyte Case Studies

8.3.2 Replicating Skeletal Muscle Structure In Vitro Using Topographic Patterns for Disease Modelling and Drug Screening Applications

8.3.3 Other Applications: Patterned Smooth Muscle Cells for Vascular Modelling and Tissue Engineering

8.4 Future Directions

8.4.1 Topography Is Only a Piece of the Maturation Puzzle

8.4.2 High-throughput Assays and Assay Agnostic Applications

8.4.3 Fit for Purpose?

8.4.4 Three-dimensional Applications

8.5 Conclusions

Disclosures

References.

Chapter 9 Engineered Substrates with Dynamically Tunable Topography

9.1 Topography

9.2 Silicone Elastomer System for Micro- and Nanofabrication

9.2.1 Design of Lithography-free Reconfigurable Wrinkles Using PDMS

9.2.2 PDMS with Reconfigurable Topography to Guide Cell Functions

9.3 Surface Relief Grating System

9.3.1 Photoinduced Surface Relief as Reconfigurable Topography

9.3.2 Reconfigurable Topography Based on PSR for Guiding Cell Functions

9.4 Shape-memory Polymer System

9.4.1 Design of Programmed Topography Change Based on SMPs

9.4.2 SMP Platforms with Programmable Topography for Guiding Cell Functions

9.5 Conclusions and Future Perspective

Chapter 10 Curvature Mechanobiology

10.1 Introduction

10.1.1 Motivation to Investigate Cellular Behaviours on Curved Surfaces

10.1.2 Curvature as a Fundamental Geometrical Parameter

10.2 Engineering Curved Surface

10.2.1 Basic Moulding Techniques

10.2.2 Controlling Interfacial Geometries

10.2.3 Machining and Etching

10.2.4 Lithography

10.2.5 Technical Perspectives

10.3 Biomolecules Related to Cellular Curvature Sensing

10.3.1 What Senses Cellular-scale Curvature?

10.3.2 Curvature Recognition viaMolecular Affinity

10.3.3 Role of Intracellular Components

10.3.4 Role of Intercellular Connections

10.3.5 Current Perspectives

10.4 Modelling Cellular Behaviours on Curved Surfaces

10.4.1 Continuum Modelling Approaches

10.4.2 Discrete Modelling Approaches

10.4.3 Future Subjects

10.5 Conclusions

Chapter 11 Dynamic Hydrogel

11.1 Hydrogel Classification and Switchable Hydrogel Design Principles

11.2 Hydrogels Exhibiting One-way Stiffness Changes

11.2.1 Design of Switchable Hydrogels with Decreasable Elastic Modulus.

11.2.2 Design of Switchable Hydrogels with Increasable Elastic Modulus

11.3 Hydrogels Capable of Reversible Stiffness Changes

11.3.1 Reversible Stiffness Changes in Response to Chemical Stimuli

11.3.2 Reversible Stiffness Changes in Response to Physical Stimuli

11.4 Cell Scaffolds Capable of Stress Relaxation

11.5 Conclusions and Future Perspectives

Chapter 12 Manipulation of Durotaxis on a Matrix with Cell-scale Stiffness Heterogeneity

12.1 Introduction

12.2 Threshold Stiffness Gradient Required to Induce Durotaxis

12.2.1 Fabrication of a Stiffness Gradient Substrate

12.2.2 Determination of the Threshold Stiffness Gradient Required to Induce Durotaxis

12.3 Manipulation of Durotaxis

12.3.1 Rectified Durotaxis

12.3.2 Reverse Durotaxis

12.4 Combined Durotaxis-induced Nomadic Cell Migration

12.5 Conclusions and Perspectives

Chapter 13 Engineered 3D Matrices with Spatiotemporally Tunable Properties

13.1 Introduction

13.2 The Importance of 3D Cell Culture

13.3 Design Strategy of Engineered Matrices for 3D Cell Culture

13.3.1 Azide-Alkyne Cycloaddition for Engineered Hydrogel Crosslinking

13.3.2 Diels-Alder Reaction for Engineered Hydrogel Crosslinking

13.3.3 Thiol-Ene Reaction for Engineered Hydrogel Crosslinking

13.3.4 Other Reactions for Engineered Hydrogel Crosslinking

13.4 Engineered Matrices for 4D Cell Culture

13.4.1 Programmable Hydrogels Based on Degradability

13.4.2 Engineered 3D Matrices with Spatiotemporally Programmable Biochemical Cues

13.4.3 Adaptable Engineered 3D Matrices for Mechanobiology

13.4.4 Conclusions and Future Perspective

Chapter 14 Combining Genetic and Mechanical Factors to Model Disease

14.1 Introduction

14.2 Modeling Disease with a Single Variable.

14.2.1 Genetic Models.

Notes:

Description based on publisher supplied metadata and other sources.

Description based on print version record.

Includes bibliographical references and index.

ISBN:

9781839165382

1839165383

9781839165375

1839165375