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Biochemical Pathways and Environmental Responses in Plants : Part B / edited by Joseph Jez.
- Format:
- Book
- Series:
- Methods in enzymology ; Volume 680.
- Methods in enzymology, ; Volume 680
- Language:
- English
- Subjects (All):
- Enzymology.
- Plants--Metabolism.
- Plants.
- Physical Description:
- 1 online resource (xviii, 491 pages) : illustrations (chiefly color).
- Other Title:
- Biochemical Pathways and Environmental Responses in Plants
- Place of Publication:
- Cambridge : Academic Press, 2023.
- Summary:
- Biochemical Pathways and Environmental Responses in Plants, Part B, Volume 682 in the Methods in Enzymology series, highlights advances in the field with this new volume presenting chapters on MIE 681/682: Biochemical pathways and environmental responses in plants, Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, Biochemical characterization of plant aromatic aminotransferases, and much more. Other chapters focus on Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.
- Contents:
- Chapter One - Systems and strategies for plant protein expression
- Chapter Two - Biochemical characterization of plant aromatic aminotransferases
- Chapter Three - Assaying plant formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based method
- Chapter Four - Functional analysis of phosphoethanolamine N-methyltransferase in plants and parasites: Essential S-adenosylmethionine-dependent methyltransferase in choline and phospholipid metabolism
- Chapter Five - An approach to nearest neighbor analysis of pigment-protein complexes using chemical cross-linking in combination with mass spectrometry
- Chapter Six - Structural characterization of protein-DNA complexes using small angle X-ray scattering (SAXS) with contrast variation
- Chapter Seven - A fast and cost-effective procedure for reliable measurement of trypsin inhibitor activity in soy and soy products
- Chapter Eight - Strategies to study the metabolic origins of specialized plant metabolites: The specialized 1,4-naphthoquinones
- Chapter Nine - A liquid chromatography-mass spectrometry-based metabolomics strategy to explore plant metabolic diversity
- Chapter Ten - Quantification of plant cardenolides by HPLC, measurement of Na+/K+-ATPase inhibition activity, and characterization of target enzymes
- Chapter Eleven - Ex vivo metabolomics-A hypothesis-free approach to identify native substrate(s) and product(s) of orphan enzymes
- Chapter Twelve - Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation
- Chapter Thirteen - Controlled environments for cannabis cultivation to support "omics" research studies and production
- Chapter Fourteen - Standard operating procedures for the comprehensive and reliable analysis of cannabis terpenes
- Chapter Fifteen - Isolation of novel chemical components and their plant target proteins under selenium stress
- Chapter Sixteen - Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing
- Chapter Seventeen - Analysis of plant flooding response.
- Notes:
- Includes bibliographical references.
- Description based on publisher supplied metadata and other sources.
- ISBN:
- 9780443185847
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