Basic and advanced laboratory techniques in histopathology and cytology / Pranab Dey.

Format:

Book

Author/Creator:

Dey, Pranab, author.

Language:

English

Subjects (All):

Cytology--Technique.

Histology, Pathological--Technique.

Histology, Pathological.

Cytology.

Medical laboratory technology.

Histology--Technique.

Histology.

Physical Description:

1 online resource (xxix, 340 pages) : illustrations (chiefly color)

Edition:

Second edition.

Place of Publication:

Singapore : Springer, [2022]

Summary:

The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

Contents:

Chapter 1: Fixation of histology samples: Principles, methods and types of fixatives

Chapter 2: Processing of tissue in the histopathology laboratory

Chapter 3: Embedding of tissue in histopathology

Chapter 4: Decalcification of bony and hard tissue for histopathology processing

Chapter 5: Tissue Microtomy: principle and procedure

Chapter 6: Frozen section: Principle and procedure

Chapter 7: Staining principle and general procedure of staining of the tissue

Chapter 8: Haematoxylin and Eosin stain of the tissue section

Chapter 9: Special Stains for the carbohydrate, protein, lipid, nucleic acid and pigments

Chapter 10: Connective tissue stain: Principle and procedure

Chapter 11: Amyloid staining

Chapter 12: Stains for the Microbial Organisms

Chapter 13: Cytology sample procurement, fixation and processing

Chapter 14: Routine staining in cytology laboratory

Chapter 15: The basic technique of fine needle aspiration cytology

Chapter 16: Immunocytochemistry in histology and cytology

Chapter 17: Flow cytometry: Basic principles, procedure and applications in pathology

Chapter 18: Digital pathology

Chapter 19: Automation in the laboratory and Liquid-based cytology

Chapter 20: Polymerase chain reaction: Principle, technique and applications in pathology

Chapter 21: Fluorescent in situ hybridisation techniques in pathology: Principle, technique and applications

Chapter 22: Tissue microarray in pathology: Principal, technique and applications

Chapter 23: Sanger sequencing and next-generation gene sequencing: Basic principles and applications in pathology

Chapter 24: Liquid biopsy: Basic principles, techniques and applications

Chapter 25: Artificial neural network in Pathology: Basic principles and applications

Chapter 26: Compound Light microscope and other different microscopes

Chapter 27: Fluorescence microscope, confocal microscope and other advanced microscopes: Basic principles and applications in pathology

Chapter 28: Electron microscopy: Principle, components, optics and specimen processing

Chapter 29: Quality control and laboratory organization

Chapter 30: Laboratory safety and laboratory waste disposal

Multiple choice questions for the self-assessment.

Notes:

Includes bibliographical references.

Print version record.

Other Format:

Print version: Dey, Pranab. Basic and advanced laboratory techniques in histopathology and cytology.

ISBN:

9789811966163

9811966168

OCLC:

1365391016

Access Restriction:

Restricted for use by site license.