CCN Proteins : Methods and Protocols / edited by Masaharu Takigawa.

Format:

Book

Contributor:

Takigawa, Masaharu, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2582

Methods in Molecular Biology, 1940-6029 ; 2582

Language:

English

Subjects (All):

Proteins.

Cytology.

Cell Biology.

Local Subjects:

Proteins.

Cell Biology.

Physical Description:

1 online resource (XIV, 430 pages) : 86 illustrations, 66 illustrations in color.

Edition:

2nd edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.

Contents:

CCN Proteins - Cellular Communication Network Factors: Expanding their Repertoire toward a New Concept

Optimal CCN4 Immunofluorescence for Tissue Microarray

Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes

Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-free Fractions, and Cells

Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes

Evaluation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance

Protocols for Screening Peptides Binding to CCN Family Proteins and their Extended Utility

Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism

Analyses of the Post-Transcriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression

Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network

Utilizing Public Molecular Biological Databases for CCN Family Research

Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation

Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy

The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues

Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis

Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins

Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells

Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo

Angiogenesis Assays for the Analysis of CCN Proteins

Using the Bleomycin-Induced Model of Fibrosis to Study the Contribution of CCN Proteins to Scleroderma Fibrosis

Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1

Immunohistochemical Analysis of CCN2 in Experimental Fracture Healing Models

Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins

Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products

Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain and Loss of Function Approaches

Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney

Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis.

Other Format:

Printed edition:

ISBN:

978-1-0716-2744-0

9781071627440

Access Restriction:

Restricted for use by site license.