SILAC : Methods and Protocols / edited by Jose L. Luque-Garcia.

Format:

Book

Contributor:

Luque-Garcia, Jose L., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2603

Methods in Molecular Biology, 1940-6029 ; 2603

Language:

English

Subjects (All):

Proteins.

Cytology-Technique.

Cytological Techniques.

Local Subjects:

Proteins.

Cytological Techniques.

Physical Description:

1 online resource (XII, 286 pages) : 41 illustrations, 31 illustrations in color.

Edition:

1st edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Contents:

Dynamic SILAC to Determine Protein Turnover in Neurons and Glia

SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe

Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics

pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles

Quantification of Protein Palmitoylation by Cysteine-SILAC

Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC)

A Super-SILAC Approach for Profiling Histone Post-Translational Modifications

SILAC-Based Quantitative Phosphoproteomics in Yeast

Phosphotyrosine Profiling Using SILAC

Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins

Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules

Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC

Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons

Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae

Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Proteins Using SILAC

Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions

Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC

Using SILAC to Develop Quantitative Data Independent Acquisition (DIA) Proteomics Methods

SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status

Targeted Absolute Protein Quantification Using SILAC Internal Standard and Full-Length Protein Calibrators (TAQSI).

Other Format:

Printed edition:

ISBN:

978-1-0716-2863-8

9781071628638

Access Restriction:

Restricted for use by site license.