1 option
T-Cell Development : Methods and Protocols / edited by Remy Bosselut, Melanie S. Vacchio.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2580
- Methods in Molecular Biology, 1940-6029 ; 2580
- Language:
- English
- Subjects (All):
- Immunology.
- Cancer.
- Cancers.
- Local Subjects:
- Immunology.
- Cancers.
- Physical Description:
- 1 online resource (XI, 384 pages) : 76 illustrations, 41 illustrations in color.
- Edition:
- 2nd edition 2023.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2023.
- System Details:
- text file PDF
- Summary:
- This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.
- Contents:
- A beginner's guide to T cell development
- Revelations in thymic epithelial cell biology and heterogeneity from single cell RNA sequencing and lineage tracing methodologies
- Early development of innate lymphoid cells
- Development of gd T cells, Soldiers on the Front Lines of Immune Battles
- Principles of Advanced Flow Cytometry: A Practical Guide
- Genetic strategies to study T cell development
- A simplified approach to evaluating T cell development by flow cytometry
- Purification of thymocyte and T cell subsets
- Generation of bone marrow chimeras
- Generation of murine T cell effector populations in vitro
- Large-scale isolation of mouse thymic epithelial cells
- Generation of retrogenic mice to investigate T cell developmentPurification of bone marrow precursors to T cells and ILCs
- Studying T cell Development in Neonatal and Adult Thymic Slices
- Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines
- Methods for Study of Mouse T Cell Receptor a and b Gene Rearrangements
- Ultrasound guided intra-thymic cell injection
- Fetal thymic organ culture and negative selection
- In Vitro Analysis of Thymocyte Signaling
- Identification and purification of human T cell precursors
- In vitro model systems to study human T cell development
- Zebrafish: A Tractable Model for Analysis of T cell Development. .
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-2740-2
- 9781071627402
- Access Restriction:
- Restricted for use by site license.
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.