Chemokine-Glycosaminoglycan Interactions : Methods and Protocols / edited by Alexandra R. Lucas.

Format:

Book

Contributor:

Lucas, Alexandra R., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2597

Methods in Molecular Biology, 1940-6029 ; 2597

Language:

English

Subjects (All):

Immunology.

Cytology.

Cell Biology.

Local Subjects:

Immunology.

Cell Biology.

Physical Description:

1 online resource (XV, 262 pages) : 1 illustrations

Edition:

1st edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.

Contents:

A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy

Remote Ischemic Pre-Conditioning (RIPC)

Mouse Corneal Transplantation

Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant

Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade

Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion

In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor

The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins

Flow Cytometry Analysis of Immune Cell Responses

Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence

Protein Microarrays and Their Fabrication

NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions

Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions

Isolation and Compositional Analysis of Glycosaminoglycans

How to Design Peptides

Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins

Producing Biologics with Defined N-Glycosylation in Plants

Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery.

Other Format:

Printed edition:

ISBN:

978-1-0716-2835-5

9781071628355

Access Restriction:

Restricted for use by site license.