Plant Proteostasis : Methods and Protocols / edited by L. Maria Lois, Marco Trujillo.

Format:

Book

Contributor:

Lois, L. Maria, Editor.

Trujillo, Marco, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2581

Methods in Molecular Biology, 1940-6029 ; 2581

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XVI, 415 pages) : 80 illustrations, 57 illustrations in color.

Edition:

2nd edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field. .

Contents:

Observing real-time ubiquitination in high throughput with fluorescence polarization

Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating-Enzymes and E3 Ubiquitin Ligases

Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis thaliana seedlings

An in vitro assay to recapitulate hormone-triggered and SCF-mediated protein ubiquitylation

Analysis of proteasome-associated ubiquitin ligase activity

Measuring the DUB Activity of Arabidopsis Deubiquitylating enzymes

SUMO conjugation and SUMO chain formation by plant enzymes

Kinetic analysis of plant SUMO conjugation machinery

Expression, purification, and enzymatic analysis of plant SUMO proteases

Monitoring autophagic flux in the model single-celled microalga Chlamydomonas reinhardtii

Detection of autophagy in plants by fluorescence microscopy

Characterization of ATG8-family interactors by Isothermal Titration Calorimetry

Protocols for studying protein stability in a Arabidopsis protoplast transient expression system

Relative Protein Lifetime Measurement in Plants using Tandem Fluorescent Protein Timers

Detection and Quantification of Protein Aggregates In Plants

Using intrinsic fluorescence to measure protein stability upon thermal and chemical denaturation

Purification and detection of ubiquitinated plant proteins using Tandem Ubiquitin Binding Entities

Titanium oxide-based phosphopeptide enrichment from Arabidopsis seedlings

Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea

Chromatin Enrichment for Proteomics in Plants (ChEP-P)

Proximity-dependent in vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha

Tandem mass tag-based phospho-proteomics in plants

Analysis of peptide hormone maturation and processing specificity using isotope-labeled peptides

Improved identification of protease cleavage sites by in-gel reductive dimethylation

A Pipeline to Monitor Proteasome Homeostasis in Plants

Bioinformatics tools for exploring the SUMO Gene Network, an update

Coexpression network constriction and visualization from transcriptomes underlying ER stress responses

Approaches for the identification of intrinsically disordered protein domains.

Other Format:

Printed edition:

ISBN:

978-1-0716-2784-6

9781071627846

Access Restriction:

Restricted for use by site license.