Retinitis Pigmentosa / edited by Stephen H. Tsang, Peter M.J. Quinn.

Format:

Book

Contributor:

Tsang, Stephen H., Editor.

Quinn, Peter M.J., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2560

Methods in Molecular Biology, 1940-6029 ; 2560

Language:

English

Subjects (All):

Medicine-Research.

Biology-Research.

Ophthalmology.

Biomedical Research.

Local Subjects:

Biomedical Research.

Ophthalmology.

Physical Description:

1 online resource (XVIII, 404 pages) : 100 illustrations, 40 illustrations in color.

Edition:

1st edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This volume details the history of Retinitis Pigmentosa and current treatment options. Chapters guide readers through CRISPR, gene therapy, stem cell therapy, next-generation sequencing methods, gene editing, and translational applications of other therapies to the treatment of Retinitis Pigmentosa. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinitis Pigmentosa aims to be a useful practical guide to researches to help further their study in this field. .

Contents:

Introduction and History of Retinitis Pigmentosa

Etiology of Retinitis Pigmentosa

Clinical Evaluation of Patients with Retinitis Pigmentosa

Molecular Genetic Testing Approaches for Retinitis Pigmentosa

Pedigree Analysis of Families and Patients Affected by Retinitis Pigmentosa

Whole Genome Sequencing for Detection of Structural Variants in Patients with Retinitis Pigmentosa

Fundus Imaging Methodologies to Assess RP patients

The Use of Optical Coherence Tomography in Evaluation of Retinitis Pigmentosa

Optical Coherence Tomography Angiography (OCTA) findings in Retinitis Pigmentosa

ERG to Evaluate the Retina in Cases of Retinitis Pigmentosa

Visual Function Tests: Visual Fields

Visual Function Tests

Visual Acuity as a Measurement of Visual Function

Measure of Visual Function

Ophthalmic Fluorescein Angiography

Indocyanine Green Angiography

Use of the Medmont Dark-Adapted Chromatic Perimeter for Assessing Rod Function in Retinitis Pigmentosa

Multi-Luminance Mobility Testing Endpoint

Preclinical Models of Retinitis Pigmentosa

ERG to evaluate the retina using mouse models

Autofluorescence Imaging to Evaluate Retinal Disease Progression in Rodent

Large animal models of retinitis pigmentosa in therapy development and preclinical testing

CRISPR manipulations in stem cell lines

CRISPR-mediated genome engineering in cell lines

CRISPR off-targeting

Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV-Mediated Gene Delivery

Culture of Human Retinal Explants for Ex Vivo Assessment of AAV Gene Delivery

Prime Editing for the Installation and Correction of Mutations causing Inherited Retinal Disease: A Brief Methodology

Large scale single-nucleus RNA sequencing compatible with complex archived samples

Vector Shedding and Immunogenicity Sampling in AAV-based Gene Therapy for Retinitis Pigmentosa Patients

Current Management Options for Patients with Retinitis Pigmentosa

Challenges of Treatment Methodologies and the Future of Gene Therapy and Stem Cell Therapy to Treat Retinitis Pigmentosa

Ocular Injection Techniques for Retinitis Pigmentosa: Intravitreal, Subretinal, and Suprachoroidal

Surgical Approach with Pars Plana Vitrectomy for Subretinal Gene Therapy.

Other Format:

Printed edition:

ISBN:

978-1-0716-2651-1

9781071626511

Access Restriction:

Restricted for use by site license.