Golgi : Methods and Protocols / edited by Yanzhuang Wang, Vladimir V Lupashin, Todd R. Graham.

Format:

Book

Contributor:

Wang, Yanzhuang, Editor.

Lupashin, Vladimir V., Editor.

Graham, Todd R., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2557

Methods in Molecular Biology, 1940-6029 ; 2557

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XVII, 816 pages) : 196 illustrations, 167 illustrations in color.

Edition:

1st edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.

Contents:

Live Cell Imaging of Yeast Golgi Dynamics

Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuole

Immunofluorescence as a Method to Study Golgi Organization in Larval Salivary Glands of Drosophila melanogaster

Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgi

Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells

Generation of GM130 Conditional Knockout Mouse

Monitoring Effects of Membrane Traffic via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts

Immunofluorescence Microscopy of the Mammalian Golgi Apparatus

Studying the Organization of the Golgi by Super-Resolution Microscopy

Super-Resolution Live Imaging of Cargo Traffic Through the Golgi Apparatus in Mammalian Cells

Studying Golgi Structure and Function by Thin Section TEM

Algorithm for Modern Electron Microscopic Examination of the Golgi Complex

High-Pressure Freezing Followed by Freeze-Substitution, An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking

Negative Staining Electron Microscopy for Morpho-Functional Characterization of Purified Golgi Membranes from Mammalian Cells

An Electron Tomographic Analysis of Giantin Deficient Golgi Proposes a New Function of the Golgin Protein Family

Modeling the Cryo-EM Structure of the Exocyst Complex

Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Software

A Primer on Deep Learning-Based Cellular Image Classification of Changes in the Spatial Distribution of the Golgi Apparatus After Experimental Manipulation

Reconstituting Vesicle Budding from the TGN and Immunoisolation of Vesicles Enriched with a Specific Cargo Client

Common Assays in Mammalian Golgi Studies

In Vitro Methods to Investigate the Disassembly of the Golgi Ribbon during the G2-M Transition of the Cell Cycle

Generation and Analysis of hTERT-RPE1 VPS54 Knock-Out and Rescued Cell Lines

Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System

Generation of Stable Cell Lines Expressing Golgi Reassembly Stacking Proteins (GRASPs) by Viral Transduction

Quantitative Proteomics Analysis of Purified Rat Liver Golgi

Comparative Genomics for Evolutionary Cell Biology using AMOEBAE: Understanding the Golgi and Beyond

Common Markers and Small Molecule Inhibitors in Golgi Studies

Visualizing Reversible Cisternal Stacking in Budding Yeast Pichia pastoris

Methods for Studying Membrane-Proximal GAP Activity on Prenylated Rab GTPase Substrates

Studying the Role of Lipid Geometry in COPI Vesicle Formation

Unbiased Quantification of Golgi Scattering and Golgi-Centrosome Association

Detection and Analysis of Microtubule Nucleator γ-Tubulin Ring Complex

Quantification of Golgi Entry and Exit Kinetics of Protein Cargoes

CARTS Formation Assay

Quantification of Protein Exit at the Trans-Golgi Network

Endosomal Transport to Lysosomes and the Trans-Golgi Network in Neurons and Other Cells: Visualizing Maturational Flux

Immunoprecipitation and Western Blot Analysis of AP-1 Clathrin-Coated Vesicles

Live imaging of Golgi Outposts in Drosophila Dendritic Arbors

Targeted Protein Unfolding at the Golgi Apparatus

Modeling N-Glycosylation: A Systems Biology Approach for Evaluating Changes in the Steady State Organization of Golgi Resident Proteins

Simple N-Glycan Profile Analysis Using Lectin Staining, Mass Spectrometry, and GlycoMaple

In Vitro Methods to Study the Golgi Apparatus Role in Proteoglycan and Glycosaminoglycan Synthesis

Analysis of Golgi Protein Acetylation using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry

Identification and Characterization of GRASP55 O-GlcNAcylation

Assaying Sterol-Regulated ER-to-Golgi Transport of SREBP Cleavage-Activating Protein Using Immunofluorescence Microscopy

Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software

Analysis of Golgi Secretory Functions in Cancer.

Other Format:

Printed edition:

ISBN:

978-1-0716-2639-9

9781071626399

Access Restriction:

Restricted for use by site license.