Microbial Environmental Genomics (MEG) / edited by Francis Martin, Stephane Uroz.

Format:

Book

Contributor:

Martin, Francis, Editor.

Uroz, Stéphane, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2605

Methods in Molecular Biology, 1940-6029 ; 2605

Language:

English

Subjects (All):

Microbial genetics.

Microbiology.

Microbial Genetics.

Local Subjects:

Microbial Genetics.

Microbiology.

Physical Description:

1 online resource (XI, 365 pages) : 88 illustrations, 71 illustrations in color.

Edition:

2nd edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This volume guides researchers on how to characterize, image rare, and hitherto unknown taxa and their interactions, to identify new functions and biomolecules and to understand how environmental changes condition the activity and the response of the organisms living with us and in our environment. Chapters cover different organism types (id est, archaea, bacteria, fungi, protist, microfauna and microeukaryotes) and propose detailed protocols to produce high quality DNA, to analyse active microbial communities directly involved in complex interactions or processes through stable isotope probing, to identify and characterize new functional genes, to image in situ interactions and to apply bioinformatics analysis tools to complex metagenomic or RNAseq sequence data. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial Environmental Genomics (MEG): Methods and Protocols, Second Edition aims to serve as a primary research reference for researchers in microbiology working to in the expanding field of molecular ecology and environmental genomics.

Contents:

Metabarcoding Approaches For Soil Eukaryotes, Protists and Microfauna

Metabarcoding of the three domains of life in aquatic saline ecosystems

Sample preparation for fungal community analysis by high-throughput sequencing of barcode amplicons

Characterization of seed mycobiota using culture-dependent and -independent approaches

Tissue Cultivation, Preparation, and Extraction of High Molecular Weight DNA for Single-Molecule Genome Sequencing of Plant-Associated Fungi

Analysis of ancient microbial DNA

Whole Genome Sequencing of bacterial endophytes from fresh and preserved plant specimens

Investigating the bacterial and fungal communities involved in dead biomass degradation in forest soils

Stable isotope probing-RNA strategy to study plant/fungus interactions

Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis

Construction of a transposon mutant library in the pathogen Agrobacterium tumefaciens C58 and identification of genes involved in gall niche exploitation and colonization

High-throughput screening of fosmid libraries for increased identification of novel N-acyl homoserine lactone degrading enzymes

Analyzing prokaryotic transcriptomics in the light of genome data with the MicroScope platform

MycoCosm, the JGI's Fungal Genome Portal for Comparative Genomic and Multiomics Data Analyses

Investigating endobacteria that thrive within Mucoromycota

Confocal laser scanning microscopy approach to investigate plant-fungal interactions

Visualisation of fungi during wood colonisation and decomposition by microscopy: from light to electron microscopy.

Other Format:

Printed edition:

ISBN:

978-1-0716-2871-3

9781071628713

Access Restriction:

Restricted for use by site license.