Basic and Advanced Laboratory Techniques in Histopathology and Cytology [electronic resource] / by Pranab Dey.

Format:

Book

Author/Creator:

Dey, Pranab, Author.

Language:

English

Subjects (All):

Pathology.

pathology.

Medical Subjects:

Pathology.

Local Subjects:

Pathology.

Genre:

Electronic books.

Physical Description:

1 online resource (XXV, 275 p. 174 illus. in color.)

Edition:

1st ed. 2018.

Place of Publication:

Singapore : Springer Singapore : Imprint: Springer, 2018.

Summary:

This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. In addition, it covers various advanced laboratory techniques, such as immunocytochemistry, flow cytometry, liquid based cytology, polymerase chain reaction, tissue microarray, and molecular technology. Further, the book includes numerous color illustrations, tables and boxes to familiarize the reader with the work of a pathology laboratory. The book is mainly intended for postgraduate students and fellows in pathology as well as practicing pathologists. The book is also relevant for all the laboratory technicians and students of laboratory technology.

Contents:

Fixation of histology samples

Processing of tissue

Embedding of tissue

Decalcification of bone

Tissue microtomy

Frozen section of tissue: technique and applications

Staining principle and general procedure of stain

Haematoxylin and eosin stain

Special stains for protein, lipid, carbohydrate, nucleic acid and pigments

Stains for the connective tissue

Stains for the amyloid

Stains for the microbial organisms

Cytology sample: procurement, fixation and processing

Routine staining in cytology laboratory

Fine needle aspiration biopsy technique

Immunochemistry in histology and cytology: principle, technique and applications

Flow cytometry: principle, technique and applications

Digital image analysis and virtual microscopy: principle, technique and applications

Liquid based cytology and automated screening devices

Polymerase chain reaction and DNA sequencing: principle, technique and applications

Fluorescent in situ hybridisation techniques: principle, technique and applications

Tissue microarray: technique and applications

Sanger sequencing and next generation gene sequencing: basic principles and applications

Light microscope: optical components, principle and handling

Fluorescence microscope and confocal microscopy: basic parts and principle

Laboratory organization and quality control

Laboratory safety: essential precautions.

ISBN:

9811082529

9789811082528

OCLC:

1180309126

Access Restriction:

Restricted for use by site license.