Glycovirology : Methods and Protocols / edited by Yasuo Suzuki.

Format:

Book

Contributor:

Suzuki, Yasuo, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2556

Methods in Molecular Biology, 1940-6029 ; 2556

Language:

English

Subjects (All):

Virology.

Clinical biochemistry.

Medical Biochemistry.

Local Subjects:

Virology.

Medical Biochemistry.

Physical Description:

1 online resource (XI, 357 pages) : 110 illustrations, 68 illustrations in color.

Edition:

1st ed. 2022.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2022.

System Details:

text file PDF

Summary:

This volume details applications in molecular biological techniques and focuses on applications to determine the involvement of glycans in virus interactions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glycovirology: Methods and Protocols aims to be an essential resource for researchers who wish to learn more about glycovirology. .

Contents:

Comprehensive cellular glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods

High-performance liquid chromatography mapping method for the structural analysis of N-glycans at the molecular, cellular, and tissue levels

Detection of sialic acids on the cell surface using flow cytometry

Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of Nonhuman Primates

Preparation of Glycan Arrays Using Glycopeptides Derived from Biomaterials

Glycan profiling of viral glycoproteins with the lectin microarray

Preparation of recombinant PA endonuclease domain protein of influenza A virus and its application for glycobiology research

Receptor-binding specificity of influenza viruses.-Functional analysis of sulphatide in influenza A virus infection and replication

Quantification of receptor-association, -dissociation and NA-dependent motility of influenza A particles by biolayer interferometry

Receptor-binding assay for avian influenza viruses

Characterization of influenza virus binding to receptors on isolated cell membranes

Characterization of human parainfluenza virus receptor using terminal sialic acid linkage-modified cells

Solid-phase binding assay for ganglioside-binding of human respiroviruses

Evaluation of the glycan-binding and esterase activities of hemagglutinin-esterase-fusion glycoprotein from influenza D virus

Roles of glycans and non-glycans on the epithelium and in the immune system in H1-H18 influenza A virus infections

Roles of sialyl glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 infections

Enzymatic substrates and fluorescence imaging of influenza virus sialidase

Assays for determining the sialidase activity of influenza viruses and monitoring influenza virus susceptibility to neuraminidase inhibitors

Synthesis and Neuraminidase Inhibitory Activity of Sialic Acid Analogues with Fluoro, Phosphono and Sulfo Functionalities

Influenza A virus neuraminidase inhibitors.

Other Format:

Printed edition:

ISBN:

978-1-0716-2635-1

9781071626351

Access Restriction:

Restricted for use by site license.