Dynamin Superfamily GTPases : Methods and Protocols / edited by Rajesh Ramachandran.

Format:

Book

Contributor:

Ramachandran, Rajesh, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2159

Methods in Molecular Biology, 1940-6029 ; 2159

Language:

English

Subjects (All):

Chemistry.

Local Subjects:

Chemistry.

Physical Description:

1 online resource (X, 221 pages) : 53 illustrations, 33 illustrations in color.

Edition:

1st ed. 2020.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2020.

System Details:

text file PDF

Summary:

This volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.

Contents:

Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae

Purification of the Dynamin-related Protein Vps1 using Mammalian and Bacterial Expression Systems

A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli

Affinity Purification and Functional Characterization of Dynamin-related Protein 1

Purification and Characterization of MxB

Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding

Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-lipid Interactions

Nucleotide-dependent Dimerization and Conformational Switching of Atlastin

Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System

Cell-free Analysis of Mitochondrial Fusion by Fluorescence Microscopy

Electrophysiological Methods For Detection Of Membrane Leakage and Hemifission by Dynamin 1

Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore

Cellular Assays for Measuring Dynamin Activity in Muscle Cells

Measuring Drp1 Activity in Mitochondrial Fission in vivo

Quantifying Drp1-mediated Mitochondrial Fission by Immunostaining in Fixed Cells

Imaging Dynamin-related Protein 1 (Drp1)-mediated Mitochondrial Fission in Living Cells. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0676-6

9781071606766

Access Restriction:

Restricted for use by site license.