Homologous Recombination : Methods and Protocols / edited by Andrés Aguilera, Aura Carreira.

Format:

Book

Contributor:

Aguilera, Andrés, Editor.

Carreira, Aura, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2153

Methods in Molecular Biology, 1940-6029 ; 2153

Language:

English

Subjects (All):

Medical genetics.

Medical Genetics.

Local Subjects:

Medical Genetics.

Physical Description:

1 online resource (XV, 557 pages) : 126 illustrations, 91 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume explores homologous recombination's (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. .

Contents:

Detection of DNA Double Strand Breaks by γ-H2AX Immunodetection

END-seq: An Unbiased, High-Resolution and Genome-Wide Approach to Map DNA Double Strand Breaks and Resection in Human Cells

Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting

Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe

Quantifying DNA End Resection in Human Cells

Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure-Forming Repeats

Biochemical analysis of D-Loop Extension and DNA Strand Displacement Synthesis

DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing

Monitoring Homologous Recombination Activity in Human Cells

Inter-Homolog Homologous Recombination in Mouse Embryonic Stem Cells

Branch Migration Activity of Rad54 Protein

Holliday Junction Resolution

Identification and Analysis of Different Types of UFBs

Intrachromosomal Recombination in Yeast

Genome-Wide Analysis of Mitotic Recombination in Budding Yeast

Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis

DNA Double-Strand Break Induced Gene Amplification in Yeast

Measuring Chromosome Pairing during Homologous Recombination in Yeast

Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes

Detection of DSBs in C. elegans Meiosis

Methods to Map Meiotic Recombination Proteins in Saccharomyces cerevisiae

Investigation of Break-Induced Replication in Yeast

Measurement of Homologous Recombination at Stalled Mammalian Replication Forks

Super Resolution Imaging of Homologous Recombination Repair at Collapsed Replication Forks

The Analysis of Recombination-Dependent Processing of Blocked Replication Forks by Bi-Dimensional Gel Electrophoresis

The Sister Chromatid Exchange Assay in Human Cells

Analysis of Recombination at Yeast Telomeres

Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae

Analyzing Homologous Recombination at a Genome Wide Level

CRISPR/Cas9 Induced Breaks in Heterochromatin, Visualized by Immunofluorescence

In Vivo Binding of Recombination Proteins of Non-DSB DNA Lesions to Replication Forks

Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells

In Vitro Characterization of Sumoylation of HR Proteins

High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products

Fluorescence Microscopy for Analysis of Relocalization of Structure-Specific Endonucleases

Physical and Genetic Assays for the Study of DNA Joint Molecules Metabolism and Multi-Invasion-Induced Rearrangements in S. cerevisiae.

Other Format:

Printed edition:

ISBN:

978-1-0716-0644-5

9781071606445

Access Restriction:

Restricted for use by site license.