Nanopore Technology : Methods and Protocols / edited by Monifa A.V. Fahie.

Format:

Book

Contributor:

Fahie, Monifa A.V., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2186

Methods in Molecular Biology, 1940-6029 ; 2186

Language:

English

Subjects (All):

Proteins.

Biophysics.

Biotechnology.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Biophysics.

Biotechnology.

Physical Description:

1 online resource (X, 231 pages) : 57 illustrations, 43 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.

Contents:

Preparation of Fragaceatoxin C (FraC) Nanopores

Preparation of Cytolysin A (ClyA) Nanopores

Building Synthetic Transmembrane Peptide Pores

Design and Assembly of Membrane-Spanning DNA Nanopores

Determining the Orientation of Porins in Planar Lipid Bilayers

Revelation of Function and Inhibition of Wza through Single Channel Studies

Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore

Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules

A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore

Oligonucleotide-Directed Protein Threading through a Rigid Nanopore

Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP

Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel

Free Energy Minimization for Vesicle Translocation through a Narrow Pore

Single Ion-Channel Analysis in Droplet Interface Bilayer

Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer

Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.

Other Format:

Printed edition:

ISBN:

978-1-0716-0806-7

9781071608067

Access Restriction:

Restricted for use by site license.