The Epithelial-to Mesenchymal Transition : Methods and Protocols / edited by Kyra Campbell, Eric Theveneau.

Format:

Book

Contributor:

Campbell, Kyra, Editor.

Theveneau, Eric, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2179

Methods in Molecular Biology, 1940-6029 ; 2179

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XV, 429 pages) : 85 illustrations, 79 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.

Contents:

A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition

Perspective on Epithelial Mesenchymal Transitions in Embryos

EMT, One of Many Morphological Transitions in Cellular Phase Space

Are you Interested or Afraid of Working on EMT?

Partial EMT/MET: An Army of One

EMT; An Update

Mesenchymal to Epithelial Transitions in Development and Cancer

Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos

Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration

Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo

Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors

Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging

Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo

Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster

4D Live-Imaging and Analysis of Chick Embryo Somites

In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation

Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos

Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering

CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay

Using Xenopus Neural Crest Explants to Study Epithelial-mesenchymal Transition

Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition

Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition

Methodologies for Following EMT in vivo at Single Cell Resolution

Isolation and Identification of EMT Subtypes

Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer

Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells

Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling

Mathematical Modeling of Plasticity and Heterogeneity in EMT

Control of Cell Migration Using Optogenetics. .

Other Format:

Printed edition:

ISBN:

978-1-0716-0779-4

9781071607794

Access Restriction:

Restricted for use by site license.