Nuclear Reprogramming : Methods and Protocols / edited by Kejin Hu.

Format:

Book

Contributor:

Hu, Kejin, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2239

Methods in Molecular Biology, 1940-6029 ; 2239

Language:

English

Subjects (All):

Biomaterials.

Nucleic acids.

Stem cells.

Nucleic Acid.

Stem Cell Biology.

Local Subjects:

Nucleic Acid.

Stem Cell Biology.

Physical Description:

1 online resource (XI, 306 pages) : 73 illustrations, 54 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field. .

Contents:

Pig Cloning Using Somatic Cell Nuclear Transfer

Cloning of Monkeys by Somatic Cell Nuclear Transfer

Production of Cardiomyocyte-like cells by Fibroblast Reprogramming with Defined Factors

Production of Cardiomyocytes by microRNA-mediated Reprogramming In Optimized Reprogramming Media

Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Embodied Episomal Vectors for Rapid Selection of Exogenous DNA-free Cells

Generation of Human Neurons by microRNA-mediated Direct Conversion of Dermal Fibroblasts

Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-marked Lentiviral Vectors in the Chemically Defined Medium

Episomal Reprogramming Of Human Peripheral Blood Mononuclear Cells Into Pluripotency

Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells

Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling

Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA

Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators

Reprogramming Porcine Fibroblast to EPSCs

Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance

Evaluate Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers

G-banded Karyotyping of Human Pluripotent Stem Cell Cultures

Become Competent in Generating RNA-seq Heat Maps in One Day for Novices without prior R Experience.

Other Format:

Printed edition:

ISBN:

978-1-0716-1084-8

9781071610848

Access Restriction:

Restricted for use by site license.