Myeloid-Derived Suppressor Cells / edited by Sven Brandau, Anca Dorhoi.

Format:

Book

Contributor:

Brandau, Sven, Editor.

Dorhoi, Anca, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2236

Methods in Molecular Biology, 1940-6029 ; 2236

Language:

English

Subjects (All):

Biochemistry.

Local Subjects:

Biochemistry.

Physical Description:

1 online resource (XII, 220 pages) : 57 illustrations, 36 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume explores various experimental approaches that can be used to investigate myeloid-derived suppressor cells (MDSC) and can be useful to understand the characterization of these cells. The chapters in this book cover topics such as the study of MDSC in human patient samples; employing flow cytometry or magnetic enrichment; immunophenotyping using multi-parameter flow cytometry and mass cytometry; functional characterization of MDSC by biochemical, immunological, and microscopy approaches; MDSC characterization through the use of high-resolution genomics; and novel methods of high-resolution visualization of living tissue and the purification of granulocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Myeloid-Derived Suppressor Cells: Methods and Protocols is a valuable resource for novices in this field to help them start their research on MDSC, and experienced investigators looking to establish new protocols in their lab. This book is great for anyone working in the field of biology, medicine, and veterinary medicine. .

Contents:

Immunophenotyping of Circulating Myeloid-Derived Suppressor Cells (MDSC) in the Peripheral Blood of Cancer Patients

Phenotyping of Myeloid-Derived Suppressor Cells in Nonhuman Primates

Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models

Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells

Isolation of Human Circulating Myeloid-Derived Suppressor Cells and Analysis of Their Immunosuppressive Activity

High Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry

Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models

In Vitro Generation of Human Neutrophilic Myeloid-Derived Suppressor Cells

Measuring Suppressive Activity and Autophagy in Myeloid-Derived Suppressor Cells

In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function

Analysis of Antimicrobial Activity of Monocytic Myeloid-Derived Suppressor Cells in Infection with Mycobacterium tuberculosis and Human Immunodeficiency Virus

Isolation and Functional Characterization of Myeloid-Derived Suppressor Cells in Infections Under High Containment

High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma

Single Cell Transcriptome Analysis Workflow for Splenic Myeloid-Derived Suppressor Cells from Murine Breast Cancer Models

Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia

Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.

Other Format:

Printed edition:

ISBN:

978-1-0716-1060-2

9781071610602

Access Restriction:

Restricted for use by site license.