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Crop Breeding : Genetic Improvement Methods / edited by Pasquale Tripodi.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2264
- Methods in Molecular Biology, 1940-6029 ; 2264
- Language:
- English
- Subjects (All):
- Biotechnology.
- Agriculture.
- Local Subjects:
- Biotechnology.
- Agriculture.
- Physical Description:
- 1 online resource (XI, 280 pages) : 62 illustrations, 54 illustrations in color.
- Edition:
- 1st ed. 2021.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2021.
- System Details:
- text file PDF
- Summary:
- This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
- Contents:
- Methods of Development of Biparental Mapping Populations in Horticultural Crops
- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits
- Using Metabolomics to Assist Plant Breeding
- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications
- High Resolution Melting Analysis as a Tool for Plant Species Authentication
- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods
- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome
- Association Mapping in Plants
- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs)
- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis
- RNA Interference (RNAi) in Tomato Crop Reseserch
- Protoplast Based Method for Genome Editing in Tetraploid Potato
- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria
- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant
- Assembly of TALEN and mTALE-Act for Plant Genome Engineering
- Genome Editing to Achieve the Crop Ideotype in Tomato
- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging
- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-1201-9
- 9781071612019
- Access Restriction:
- Restricted for use by site license.
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