Ewing Sarcoma : Methods and Protocols / edited by Florencia Cidre-Aranaz, Thomas G. P. Grünewald.

Format:

Book

Contributor:

Cidre-Aranaz, Florencia, Editor.

G. P. Grünewald, Thomas., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2226

Methods in Molecular Biology, 1940-6029 ; 2226

Language:

English

Subjects (All):

Cancer.

Cancer Biology.

Local Subjects:

Cancer Biology.

Physical Description:

1 online resource (XI, 337 pages) : 57 illustrations, 50 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise in vitro techniques including re-expression and functional analyses of target genes together with in vivo tools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma. A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and clearly written, Ewing sarcoma: Methods and Protocols aims to provide methods to further the study of Ewing sarcoma and bring researchers one step forward in the quest to fight this devastating disease.

Contents:

Germline Variation and Somatic Alterations in Ewing Sarcoma

Western Blot Analysis in Ewing Sarcoma

Tissue Preservation and FFPE Samples: Optimized Nucleic Acids Isolation in Ewing Sarcoma

Liquid Biopsies in Ewing Sarcoma

(Immuno-)histological Analysis of Ewing Sarcoma

Molecular Approaches to Diagnosis In Ewing Sarcoma: Fluorescence in situ hybridization (FISH)

Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR

Molecular Approaches to Diagnosis in Ewing Sarcoma: Targeted RNA Sequencing

Ewing-specific (re)expression Models

Analysis of Regulatory Sequences by Dual-luciferase Reporter Assays in Ewing Sarcoma

Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma

Drug Screening by Resazurin Colorimetry in Ewing Sarcoma

Analysis of Migration and Invasion in Ewing Sarcoma

Genetically Engineered Mouse Model in Ewing Sarcoma

Tumor Growth Analysis of Ewing Sarcoma Cell Lines Using Subcutaneous Xenografts in Mice

Metastasis Assessment in Ewing Sarcoma Using Orthotopic Xenografts

Orthotopic Implants in Mice

Ewing Sarcoma PDX Models

Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma

Main Repositories and Databases used in Ewing Sarcoma Research

Chromatin Immunoprecipitation Followed by Next-Generation Sequencing (ChIP-seq) Analysis in Ewing Sarcoma

Epigenetic Analysis in Ewing Sarcoma

Systems Biology Analysis for Ewing Sarcoma.

Other Format:

Printed edition:

ISBN:

978-1-0716-1020-6

9781071610206

Access Restriction:

Restricted for use by site license.