Small Non-Coding RNAs : Methods and Protocols / edited by Mathieu Rederstorff.

Format:

Book

Contributor:

Rederstorff, Mathieu, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2300

Methods in Molecular Biology, 1940-6029 ; 2300

Language:

English

Subjects (All):

Genetics.

Genetics and Genomics.

Local Subjects:

Genetics and Genomics.

Physical Description:

1 online resource (X, 270 pages) : 66 illustrations, 48 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

Contents:

Regulatory Non-coding RNAs: An Overview

RNA Precipitation

Quantitative and Qualitative Assessment of Small RNA Preparations

Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection

Northern Blot Detection of Tiny RNAs

Stem-loop qRT-PCR Based Quantification of miRNAs

Detection and Labeling of Small Non-coding RNAs by Splinted Ligation

Fluorescence In Situ Hybridization Of Small Non-coding RNAs

Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo

Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography

Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP)

Gene Reporter Assays to Study miRNA Function

CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo

Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs

Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma

Analysis of the RNA and protein complexome by Grad-seq

µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs

RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT

Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.

Other Format:

Printed edition:

ISBN:

978-1-0716-1386-3

9781071613863

Access Restriction:

Restricted for use by site license.