Single Stranded DNA Binding Proteins / edited by Marcos T. Oliveira.

Format:

Book

Contributor:

Oliveira, Marcos T., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2281

Methods in Molecular Biology, 1940-6029 ; 2281

Language:

English

Subjects (All):

Proteins.

Protein Biochemistry.

Local Subjects:

Protein Biochemistry.

Physical Description:

1 online resource (XI, 376 pages) : 77 illustrations, 64 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume provides a comprehensive set of protocols that can be used by any research lab to investigate diverse functional and structural properties of Single Stranded DNA Binding Proteins (SSBs) from eubacterial, archaeal, eukaryotic, mitochondrial and viral systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Stranded DNA Binding Proteins aims to be a useful practical guide to researchers to help further their study in this field. .

Contents:

The Essential, Ubiquitous Single-Stranded DNA-binding Proteins

Single-stranded DNA Binding Proteins in the Archaea

Single-molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular Complexes

Comparing SSB-PriA Functional and Physical Interactions in Gram-positive and -Negative Bacteria

In vivo binding of Single-stranded DNA-binding Protein to Stalled Replication Fork Helicases

Magnetic Tweezers-based Single Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase

High-throughput Screening to Identify Inhibitors of SSB-Protein Interactions

Single-Molecule Tethered Particle Motion Studies on the DNA Recombinase Filament Assembly and Disassembly

Generation of Fluorescent Versions of Saccharomyces cerevisiae RPA to Study the Conformational Dynamics of its ssDNA-binding Domains

RPA-1 from Leishmania sp.: Recombinant Protein Expression and Purification, Molecular Modeling and Molecular Dynamics Simulations Protocols

Single-stranded DNA Curtains for Single-molecule Visualization of Rad51-ssDNA Filament Dynamics

Following Trypanosoma cruzi RPA-DNA Interaction Using Fluorescent in Situ Hybridization Coupled with Immunofluorescence (FISH/IF)

Quantifying the affinity of Trypanosoma cruzi RPA-1 to the Single-stranded DNA Overhang of the Telomere using Surface Plasmon Resonance

Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-stranded DNA Binding Proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

Atomic Force Microscopy Reveals that the Drosophila Telomere-Capping Protein Verrocchio is a Single-stranded DNA Binding Protein

Analysis of Mitochondrial SSB-DNA Complexes and their Effects on DNA Polymerase γ Activity by Electron Microscopy and Enzymatic Assays

Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-binding Protein-DNA Complexes

Measurements of Real-time Replication Kinetics of DNA Polymerases on ssDNA Templates Coated with Single-stranded DNA-binding Proteins

Selective Suppression of Endogenous Gene Expression Using RNAi in Drosophila Schneider S2 cells

Stimulation of Variant forms of the Mitochondrial DNA Helicase Twinkle by the Mitochondrial Single-stranded DNA-binding Protein

Measuring the Complex Effects of the Single-Stranded DNA-Binding Protein gp2.5 on Primer Synthesis and Extension by the Bacteriophage T7 Replisome

Strand Displacement and Unwinding Assays to Study the Concerted Action of the DNA Polymerase and SSB during Phi29 TP-DNA rReplication

Structural Characterization of a Single-stranded DNA-binding Protein: A Case Study of the ORF6 protein from Bacteriophage Enc34. .

Other Format:

Printed edition:

ISBN:

978-1-0716-1290-3

9781071612903

Access Restriction:

Restricted for use by site license.